腐胺对白桦悬浮细胞中激素和黄酮积累影响的初步研究

为明确腐胺对白桦悬浮细胞中激素和黄酮累积的影响,在白桦悬浮细胞生长的第8天添加不同浓度的腐胺、脱落酸(ABA)、茉莉酸甲酯(MeJA)、生长素(IAA)和玉米素核苷(ZR),采用紫外分光光度计法分析白桦悬浮细胞中黄酮含量和利用酶联免疫方法测定ABA、JA、IAA和ZR含量。外源腐胺和激素的添加均不同程度地提高了白桦悬浮细胞中黄酮的累积量,其中1mmol/L腐胺处理24h时增幅最高,增加了97.90%;激素中1mg/LABA处理后增幅最高,增加了103.40%。腐胺和激素共同处理后黄酮含量的增幅均较其单独处理提高。其中1mg/L腐胺与5mg/LJA处理后黄酮含量增幅最高,比对照和腐胺处理分别增加了197.43%和103.57%。同时腐胺处理后增加了IAA和ZR的含量,而降低了ABA和JA含量。进一步分析其相关性发现,腐胺处理后激素、激素比与黄酮含量的相关性增幅不同,其中JA与黄酮含量的相关性增幅最大,由对照的-0.883变为0.903。腐胺与ABA、JA、IAA和ZR协同促进了白桦悬浮细胞中黄酮的累积。

Effect of Putrescine on Hormone and Flavonoid Accumulation in Betula platyphylla Suspension Cultured Cells

Objective]To analyze the effect of exogenous putrescine on hormone and flavonoid accumulation in Betula platyphylla suspension cultured cells.Methods]Various concentrations of putrescine and exogenous hormone were added to the eight-day-old suspension cultured cells, the content of flavonoid, ABA, JA, IAA and ZR were analyzed by UV spectrophotometer and ELISA. Results]Both Exogenous putrescine and hormone treatment increased the content of flavonoid in birch suspension cultured cells. Among of them, the highest flavonoid content was induced by 1mmol/L putrescine for 24 h, the increasing rate was 97.90%. Among of theShormoneStreatment, the highest flavonoid content was induced by 1mmol/L ABA induction for 24 h, the increasing rate was 103.40%. Both putrescine and hormone treatment increased much more flavonoid content than that of control or putrescineStreatment. The highest flavonoid content was induced by 1 mmol/L putrescine and 5 mmol/L JA , the increasing rate was 197.43% and 103.57% compared with control and putrescine treatment, respectively. At the same time, Putrescine increased the content of IAA and ZR, but decreased the conent of ABA and JA. To further analysis by correlation, we found the correlation between hormone or hormone ratio with flavonoid content was different, JA Streatment showed the highest change from -0.883 to 0.903. Conclusion]This can be inferred that the interaction between putrescineSandShormoneS(ABA,SJA,SIAASandSZR)Sregualted the flavonoid production in Betula platyphylla suspension cultured cells.