| 狍Sry基因PCR扩增的初步研究 |
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| 引用本文： | 李平,白秀娟.狍Sry基因PCR扩增的初步研究[J].经济动物学报,2005,9(2):107-109. |
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| 作者姓名： | 李平 白秀娟 |
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| 作者单位： | 东北农业大学动物科技学院,哈尔滨,150030;东北农业大学动物科技学院,哈尔滨,150030 |
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| 摘 要： | 为研究狍性别决定机制,采用聚合酶链式反应(PCR)技术对野生狍(Capreoluscapreolus)(n♀=2,n♂=2)Sry基因(哺乳动物Y染色体DNA雄性特异区)进行扩增。根据人的SRY基因核心序列设计合成了1对引物1,2。结果在野生狍雄性个体中扩增出1条带,大小约为220bp,而在雌性个体中未见扩增带,表明了Sry基因的性别特异性,为探讨野生狍的性别决定机制提供了分子资料。
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| 关 键 词： | 狍 Sry基因 聚合酶链式反应技术 |
| 文章编号： | 1007-7448(2005)02-0107-03 |
| 修稿时间： | 2005年3月22日 |
PCR Amplification of the Sry Gene of Roe Deer(Capreolus capreolus) |
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| LI Ping,BAI Xiu-juan.PCR Amplification of the Sry Gene of Roe Deer(Capreolus capreolus)[J].Journal of Economic Animal,2005,9(2):107-109. |
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| Authors: | LI Ping BAI Xiu-juan |
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| Abstract: | In order to study mechanism of sexing,the PCR technique was used to amplify the Sry gene(a male-specific DNA region on the mammalian Y chromosome) in 4 individuals of Roe deer(Capreolus capreolus).A pair of primers(1 and 2)were designed and synthesized according to the motif sequence of human Sry gene.As a result,a DNA fragment(220 bp)was amplified in male Roe deer,none DNA fragment in female Roe deer. The results showed that the Sry gene was sex-specificity and the PCR method could contribute to study mechanism of Sex determination of the Roe deer. |
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| Keywords: | roe deer Sry gene PCR amplification |
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