| 枣树植原体快速PCR检测体系研究 |
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| 引用本文： | 高静涛,牛建新,王雪莲,刘娜,叶春秀,张飞.枣树植原体快速PCR检测体系研究[J].北方园艺,2011(11):109-112. |
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| 作者姓名： | 高静涛 牛建新 王雪莲 刘娜 叶春秀 张飞 |
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| 作者单位： | 石河子大学农学院园艺系.新疆,石河子,832000;石河子大学农学院园艺系.新疆,石河子,832000;石河子大学农学院园艺系.新疆,石河子,832000;石河子大学农学院园艺系.新疆,石河子,832000;石河子大学农学院园艺系.新疆,石河子,832000;石河子大学农学院园艺系.新疆,石河子,832000 |
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| 基金项目： | 石河子大学重点科技攻关资助项目(gxjs2010-zdgg05-03) |
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| 摘 要： | 依据已获得的16S rDNA序列,通过BLAST和DNAman软件分析其同源性,找到其保守序列,并设计出NTF1/NTR1、NTF2/NTR1、NTF3/NTR1、NTF1/NTR2、NTF2/NTR2、NTF3/NTR2共5对引物序列,通过优化,筛选出了适宜的PCR检测体系,通过检测验证,该体系能很好地应用于枣疯病植原体的检测。利用优化的检测体系对新疆部分样品进行检测,发现灰枣品种的个别样品携带植原体。
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| 关 键 词： | 枣疯病 植原体 检测 |
Study the Rapid PCR Detection System of Jujube Phytoplasma |
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| GAO Jing-tao,NIU Jian-xin,WANG Xue-lian,LIU Na,YE Chun-xiu,ZHANG Fei.Study the Rapid PCR Detection System of Jujube Phytoplasma[J].Northern Horticulture,2011(11):109-112. |
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| Authors: | GAO Jing-tao NIU Jian-xin WANG Xue-lian LIU Na YE Chun-xiu ZHANG Fei |
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| Institution: | GAO Jing-tao,NIU Jian-xin,WANG Xue-lian,LIU Na,YE Chun-xiu,ZHANG Fei (Department of Horticulture,Agricultural College of Shihezi University,Shihezi,Xinjiang 832000) |
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| Abstract: | In this paper,five pairs primers(NTF1/NTR1,NTF2/NTR1,NTF3/NTR1,NTF1/NTR2,NTF2/NTR2, NTF3/NTR2)were designed according to the 16 S rDNA conservative sequence,in previous studies it was obtained and analyzed.Through compared and set the different temperature and other conditions of PCR system,the optimum reaction conditions of this PCR detection system was found,and used this method to detect some materials of Xinjiang province,and there were phytoplasma in individual samples of Grey dates.Therefore,this syst... |
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| Keywords: | jujube infected phytoplasma detect |
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