| 大蒜根尖培养诱导不定芽研究 |
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| 引用本文： | 薛寒青,王舰,周淑兰,高霞. 大蒜根尖培养诱导不定芽研究[J]. 北方园艺, 2011, 0(9): 120-122 |
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| 作者姓名： | 薛寒青 王舰 周淑兰 高霞 |
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| 作者单位： | 青海省农林科学院生物技术研究所,青海,西宁,810016;青藏高原生物技术教育部重点实验室,青海,西宁,810016;青海省农林科学院生物技术研究所,青海,西宁,810016;青藏高原生物技术教育部重点实验室,青海,西宁,810016;青海省农林科学院生物技术研究所,青海,西宁,810016;青藏高原生物技术教育部重点实验室,青海,西宁,810016;青海省农林科学院生物技术研究所,青海,西宁,810016;青藏高原生物技术教育部重点实验室,青海,西宁,810016 |
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| 基金项目： | 青海省科技厅资助项目(2006-N-515) |
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| 摘 要： | 以青海省格尔木蒜脱毒后获得的脱毒小蒜为试材,研究不同培养基、蔗糖浓度、pH值和不同浓度激素配比对脱毒的格尔木大蒜根尖诱导不定芽的影响。结果表明:最佳培养基配比为MS培养基,pH 6.2,蔗糖20 g/L,BA 2 mg/L+2,4-D 0.1 mg/L。pH为6.2时诱导率达81.7%,蔗糖浓度20 g/L时诱导率达55%,激素配比为BA 2 mg/L+2,4-D 0.1 mg/L,诱导率达55%。
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| 关 键 词： | 脱毒 大蒜 根尖 不定芽 |
Induce Adventitious Bud Garlic Root |
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| XUE Han-qing,WANG Jian,ZHOU Shu-lan,GAO Xia. Induce Adventitious Bud Garlic Root[J]. Northern Horticulture, 2011, 0(9): 120-122 |
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| Authors: | XUE Han-qing WANG Jian ZHOU Shu-lan GAO Xia |
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| Affiliation: | XUE Han-qing1,2,WANG Jian1,ZHOU Shu-lan1,GAO Xia1,2(1.Institute of Biotechnology,Qinghai Academy of Agriculture and Forestry of Science,Xining,Qinghai 810016,2.Key Laboratory Qinghai Tibet Plain Biological Technology of Education Ministry,Qinghai 810016) |
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| Abstract: | Golmud small garlic affer virus-free was used as test materials in study on effect of different medium,sucrose concentration,pH,and different concentrations of hormones on induction adventitious bud of root tip from virus-free Golmud garlic.The results showed that the best medium ratio were MS medium,pH 6.2,sucrose 20 g/L,BA 2 mg/L+2,4-D 0.1 mg/L.Induction rate was 81.7% when pH was 6.2,induction rate was 55% when sucrose concentration was 20 g/L,induced rate was 55% when the hormone ratio were BA 2 mg/L+2,... |
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| Keywords: | virus-free garlic root adventitious bud |
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