巴西橡胶两品系叶片RuBP羧化酶的免疫荧光定位

巴西橡胶树的 2个品系即 RRIM60 0和 IAN873的维管束鞘细胞富含叶绿体 ,但没有“Kranz”结构 .用从烟草提纯的Ru BP羧化酶制备兔抗 Ru BP羧化酶抗体 ,并以 FITC荧光素标记抗体 .采用直接免疫荧光法对典型的 C3植物水稻、C4 植物甘蔗和巴西橡胶树等进行了 Ru BP羧化酶的定位 .结果表明 :C3和 C4 植物叶切片中 Ru BP羧化酶的分布明显不同 ,C3植物的特异荧光存在于叶肉细胞 ,而 C4 植物的特异荧光绝大部分存在于维管束鞘细胞 .巴西橡胶树 IAN873和 RRIM60 0品系的叶肉细胞和维管束鞘细胞均存在 Ru BP羧化酶 .上述结果表明 ,巴西橡胶的一些品系 (如 IAN873、RRIM60 0 )可能属于C3-C4 中间型植物

Localization of ribulose-1, 5-bisphosphate carboxylase in leaves of two clones of Hevea brasiliensis by immunofluorescence method

The clones(RRIM600 and IAN873) of Hevea brasiliensis contain large amount of chloroplasts, but have no Kranz type. Rabbit antiserum was refined from ribulose 1, 5 bisphosphate carboxylase(RuBPCase) of tobacco, and the antibody was marked with fluoresecin isothiocyanate(FITC). RuBPCase in leaf blade transection of typical C 3, C 4 plants and Hevea brasiliensis was located by direct immunofluorescence method. The results were as follows: there was difference in the localization of RuBPcase between typical C 4 and C 3 plants, the specific fluorescence of C 3 plant(rice) existed in mesophyllous cell of chloroplasts, while the specific fluorescence of C 4 plant (sugarcane) mostly existed in bundle sheath cell of chloroplasts; The specific fluorescence of IAN873,RRIM600 was located in both mesophyllous cell and bundle sheath cell of chloroplasts, so it was concluded that the clones(IAN873,RRIM600) of Hevea brasiliensis might be belong to C 3-C 4 intermediate plant.