Effect of acaricides on the activity of a Boophilus microplus glutathione S-transferase |
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Authors: | da Silva Vaz Itabajara Torino Lermen Tiago Michelon André Sanchez Ferreira Carlos Alexandre Joaquim de Freitas Daniela Reis Termignoni Carlos Masuda Aoi |
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Institution: | Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, Av. Bento Gon?alves 9500, C.P. 15005, 91501-970 Porto Alegre, RS, Brazil. |
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Abstract: | In the present study, we report the effect of several acaricides on the enzyme activity of a Boophilus microplus recombinant glutathione S-transferase (rGST). GST was expressed in Escherichia coli and was purified with glutathione (GSH) affinity column chromatography. The kinetic constants were determined by reacting GST with the substrates 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione. We report the effect of several acaricides on the enzyme activity of rGST. Some acaricides (ethion, amitraz, chlorpyrifos, DDT, cypermethrin, diazinon, ivermectin, deltamethrin and flumethrin) inhibited rGST. Contrarily, coumaphos had an activating effect. Although the accurate mechanisms of the B. microplus resistance to acaricides remain elusive, this work helps in understanding how acaricides can interact with GST. |
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