动物园鹦鹉科鸟类H5 N1禽流感疫苗免疫后效果监测

多年来动物园虽然严格按照国家强制要求对园内饲养的野生鸟类进行禽流感疫苗的免疫接种工作,但是对于接种后各种鸟类免疫效果的问题仍未有研究。本次研究主要目的为探究圈养鸟类免疫商品化禽流感疫苗后的免疫效果如何,以及各个物种对疫苗的免疫应答水平是否相同。实验中选取动物园几种圈养小型鹦鹉科鸟类为实验对象,对其进行禽流感H5N1亚型疫苗免疫接种后,利用血凝(HA)及血凝抑制(HI)实验监测禽流感抗体水平变化规律,评估免疫效果。从初步的实验结果发现,鹦鹉科鸟类仅接种一次商品化禽流感疫苗,其免疫应答水平普遍偏低,免疫效果不理想。根据实验结果,尝试性地以虎皮鹦鹉为实验对象,尝试调整免疫程序后再次进行禽流感疫苗的免疫接种,通过多次尝试找出较好的免疫程序,使得虎皮鹦鹉的禽流感疫苗免疫效果得到改善,为今后制定出适合其他种类野生鸟类的禽流感免疫程序提供了科学依据。

Immune Effect Monitoring of H5N1 Type Avian Influenza for Some Species of Captive Psittacid Birds

Over the years,captive birds of Beijing Zoo were given the AIV vaccine immunization in strict accordance with government mandate.But the immune effects of different bird species after vaccination have not been studied.The purpose of this study was to explore the immune effects of commercial avian influenza vaccine in birds and the immune response of different species to the vaccines.Species of captive psittacid birds were selected for study.Birds were immunized with avian influenza H5N1 subtype vaccine.We then used the test of hemagglutinin（HA）and hemagglutination inhibition（HI）to monitor antibody levels and to assess immune effects.Birds immunized only one time generally showed low immune response,i.e.the immune effect was not ideal.Based on this result,we chose Budgerigar（Melopsittacus undulatus）for further study in which we adjusted the immunization procedure to gain better immune effects.This provided a scientific basis for developing immunization procedures for other species of wild birds.