| 鸡堆型艾美耳球虫特异性单抗轻链可变区基因的克隆与序列测定 |
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| 引用本文： | 康桂英,秦建华.鸡堆型艾美耳球虫特异性单抗轻链可变区基因的克隆与序列测定[J].中国畜牧兽医,2009,36(8):57-59. |
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| 作者姓名： | 康桂英 秦建华 |
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| 作者单位： | （1.内蒙古民族大学动物科学技术学院， 通辽 028000； 2.河北农业大学动物科技学院， 保定 071000） |
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| 基金项目： | 内蒙古民族大学硕士科研启动金项目 |
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| 摘 要： | 将已扩增出的鸡堆型艾美耳球虫特异性单抗轻链可变区基因进行纯化，并用纯化的基因片段与pMD-18T载体连接，将重组载体转化于感受态细胞JM109，筛选出阳性重组子，提取阳性重组子质粒并进行测序，得到特异性单抗轻链可变区的基因序列。为鸡堆型艾美耳球虫特异性单链抗体基因的构建奠定了基础。
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| 关 键 词： | 鸡堆型艾美耳球虫 单抗 可变区基因 克隆 测序 |
Cloning and Nucleotide Sequencing of Light Chain Variable Genes from Species-specific Monoclonal Antibodies against E.acervulina |
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| KANG Gui-ying,QIN Jian-hua.Cloning and Nucleotide Sequencing of Light Chain Variable Genes from Species-specific Monoclonal Antibodies against E.acervulina[J].China Animal Husbandry & Veterinary Medicine,2009,36(8):57-59. |
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| Authors: | KANG Gui-ying QIN Jian-hua |
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| Institution: | （1.College of Animal Science and Technology, Inner Mongolia University for the Nationalities, Tongliao 028000, China; 2.College of Animal Science and Technology, Hebei Agriculture University, Baoding 071000, China) |
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| Abstract: | To study the function of small molecule antibody,light chain variable genes which was amplified from species-specific monoclonal antibodies against E.acervulina was purified,and cloned into clone vector pMD18-T by gene recombination techniques.The recombinant plasmid was transformed into E.coli JM109 by screening.Several recombinants that had been inserted with the target fragments were obtained.The positive clones containing recombination were identified by the methods of the gene sequencing.The result dem... |
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| Keywords: | E acervulina monoclonal antibodies variable genes cloning nucleotide sequencing |
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