不同因子对兰州百合组培小鳞茎诱导及膨大的影响研究

为了缩短兰州百合生长周期，提高百合品质，以兰州百合组培小鳞茎为材料，研究了大量元素、NAA、6-BA、培养基状态、蔗糖、2，4-D等单一因素对百合试管鳞茎形成和膨大的影响。结果表明，高浓度的大量元素有利于鳞茎的膨大，而不利于鳞茎的诱导；大量元素浓度为2MS时鳞茎平均直径最大，但新增鳞茎数下降到4.56个；NAA浓度为0.2 mg/L时新增鳞茎数最多且鳞茎平均直径最大；6-BA浓度为0.1 mg/L时最有利于鳞茎的形成和膨大，新增鳞茎数5.25个，鳞茎平均直径10.642 mm；固体培养基对鳞茎的形成效果明显，而半固体更有利于鳞茎的膨大；蔗糖浓度为90 g/L时新增鳞茎数最多，120 g/L时鳞茎平均直径最大；2，4-D浓度0.02 mg/L处理对鳞茎的形成和膨大效果更好。

Effects of Different Factors on Bulblet Induction and Enlargement in Lanzhou Lily Bulb Tissue Cultivation

To reduce the growth period and to improve the quality of Lanzhou lily bulb, the effects of mass elements, NAA, 6-BA, medium status, sucrose and 2,4-D concentration on the formation and enlargement of small bulb of Lanzhou lily in vitro were studied. Results showed that high concentration of macro elements was beneficial to the enlargement of bulbs, but not to the induction of bulbs, the bulb diameter reached the maximum at 2MS, but the number of newly grown bulbs decreased to 4.56. When NAA concentration was 0.2 mg/L, the number of new bulbs and the bulb diameter were the highest. When 6-BA concentration was 0.1 mg/L, it was most beneficial to the formation and enlargement of bulbs, the number of new bulbs was 5.25 and the diameter of bulbs was 10.642 mg. Solid medium showed obvious effect on bulb formation, while semi-solid medium was more beneficial to bulb enlargement. When sucrose concentration was 90 g/L, the number of newly grown bulbs was the highest, the bulb diameter was the largest when sucrose concentration was 120 g/L. 0.02 mg/L 2,4-D showed better effect on bulb formation and enlargement.