Immunoprotective Efficacy of a Purified 39 kDa Nymphal Antigen of Hyalomma anatolicum anatolicum |
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Authors: | Sharma JK Ghosh S Khan MH Das G |
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Institution: | (1) Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243 122 Uttar Pradesh, India |
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Abstract: | Soluble nymphal antigens (HNAg) were purified by immunoaffinity chromatography using CNBr-activated Sepharose 4B coupled with immunoglobulin ligands from animals immunized with HNAg and 69–71% protected against challenge infestations, and 8% recovery of the purified protein (Aff-HNAg) was obtained. Following immunization of crossbred calves (Bos indicus×Bos taurus) with 1600 g of Aff-HNAg in three divided doses, significant rejections of larvae (p<0.001, 84.2%), nymphs (p<0.05, 61.4%) and adults (p<0.05, 58.7%) were recorded. No significant changes were recorded in the engorgement weights of the larvae and nymphs, but there was a significant (p<0.05) reduction in the weight of the engorged adults. Immunization conferred a significant decrease in the numbers of resultant nymphs (p<0.001) and adults (p<0.001) that had fed on the immunized animals. SDS-PAGE analysis identified a 39 kDa protein, previously isolated from larvae of Hyalomma anatolicum anatolicum, as the antigen responsible for the induction of resistance against all the stages of the tick. |
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Keywords: | antigen crossbred cattle Hyalomma anatolicum anatolicum immunoaffinity chromatography nymph protection vaccine 39 kDa protein |
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