内毒素诱导的山羊红细胞膜和肝线粒体膜Ca2+-ATP酶活性的改变及654-2的保护作用

 通过静脉注射的方法研究了大肠杆菌内毒素(ET)对山羊红细胞膜和肝粒体膜损伤的膜Ca²⁺-ATP酶活性的变化及654-2(山莨菪碱)的保护效应。结果表明,ET处理组(ⅱ组)在1h,3h红细胞膜Ca²⁺-ATP酶活性高于对照组(iv组)(p<0.05),5h有下降趋势且持续到9h.12h开始回升并低于iv组(p<0.01,p<0.05).654-2处理组(ⅲ组),Ca²⁺-ATP酶活性除在9h、12h与iv组,在3h与ⅱ组差异不显着(p>0.05)外,均高于iv组和ⅱ组(p<0.01,p<0.05).在肝线粒体中,ⅱ组Ca²⁺-ATP酶活性在5h、12h均低于iv组和ⅱ组(p<0.05,p<0.01),ⅲ组Ca²⁺-ATP酶活性均高于iv组,但12h与iv组差异不显着(p>0.05).结果提示,ET具有诱导膜Ca²⁺-ATP酶活性先激活后顿抑的效应,而654-2有明显保护膜Ca²⁺-ATP酶活性的作用。

Alteration of Membrane Ca2+-ATPase Activities of Erythrocyte, Liver Mitochondria and Protection of Anisodamine During Endotoxin-induced Injury in Goats

The alterations of membrane Ca²⁺-ATPase activities of erythrocyte,hepatic mitochondria and protection of anisodamine(654-2) in goats were studied by intravenous injection of E.Coli(O₁₁₁B₄) endotoxin(1800EU/kg body weight) and anisodamine(2.5mg/kg body weight) alone as well as in combination.The results showed that endotoxin treatment(groupⅱ) led to a marked increase of Ca²⁺-ATPase activities of erythrocyte membrane at 1h and 3h,a significant decrease of Ca²⁺-ATPase activities of erythrocyte membrane at 5h,7h,9h,12h and liver mitochondria membrane at 5h,12h in vivo,as compared to the normal control(group iv).Ca²⁺-ATPase activities in endotoxin and anisodamine coexposure group(groupⅲ) were higher than those of group ivand groupⅱ,except no significant difference at 9h,12h in erythrocyte membrane and at 12h in liver mitochondria membrane as compared to grooup ivduring all experiment.These results indicated that endotoxin could activate the membrane Ca²⁺-ATPase activities in the primary stage of administration of the endotoxin.Soon afterwards,Ca²⁺-ATPase activities were inhibited,whereas anisodamine had a prominent effect of protection on the Ca²⁺-ATPase of erythrocyte and hepatic mitochondria membrane of goats in vivo.