牛蒡子提取物体外抑菌作用的研究

用体积分数为70%的乙醇溶液提取牛蒡子,然后用滤纸片法测定提取液对病原菌的抑制作用,用二倍稀释法确定提取液的最低抑菌浓度(MIC),并进一步研究提取液的热稳定性。结果表明,牛蒡子乙醇提取液对柞蚕链球菌、金黄色葡萄球菌、番茄早疫病菌和小麦赤霉病菌等有比较明显的抑制效果,而对玉米小斑病菌和玉米弯孢病菌没有抑制作用。提取液对柞蚕链球菌的最低抑菌浓度为0.125g/L,蜡质芽孢杆菌为0.250g/L,苏云金芽孢杆菌、枯草芽孢杆菌、金黄色葡萄球菌为0.500g/L,乳酸菌为1.000g/L,大肠杆菌、番茄早疫病菌为2.000g/L,小麦赤霉病菌、柞蚕白僵病菌、葡萄白腐病菌、谷子白发病菌、烟草赤星病菌、毛霉以及水稻恶苗病菌为4.000g/L。热处理对提取物的抑菌效果无显著影响(P>0.05)。

Antifungal Activity in vitro of Arctium Ethanol Extract

The antimicrobial activities of arctium extract with 70% ethanol were studied in the paper.The result showed that the extract had significant antifungal effect against Streptococcus pernyi,Staphylococcus ureus,Alternaria solani and Fusarium graminearum,but had no effect on Helminthosporium maydis and Curvularia lunata.The MIC of extract was 0.125g/L to Streptococcus pernyi,0.250g/L to Bacillus cereus,0.500g/L to Bacillus thuringiensis,Bacillus cereus and Staphylococcus ureus,1.000g/L to Lactobacillus acidophilus,2.000g/L to Escherichia coli and Alternaria solani,and 4.000g/L to Fusarium graminearum,Beauveria bassiana,Coniella diplodiella,Sclerospora graminicola,Mucor,Alternaria alternata and Cibberella fujikuroi.Heat treatment had no significant effect on the antimicrobial activities of arctium extract.