| 三叶草愈伤组织诱导及分化的研究 |
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| 引用本文： | 王友生,王瑛,李阳春.三叶草愈伤组织诱导及分化的研究[J].草业学报,2009,18(2):212-215. |
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| 作者姓名： | 王友生 王瑛 李阳春 |
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| 作者单位： | 1.定西市农业技术推广站,甘肃 定西 743000;2.东莞植物园,广东 东莞 523086;3.甘肃农业大学草业学院,甘肃 兰州730070 |
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| 摘 要： | 以4个三叶草品种的胚轴、叶片和子叶为外植体,研究了不同激素浓度配比对愈伤组织诱导和分化的影响。结果表明,不同品种不同外植体愈伤诱导率差异较大,杂三叶下胚轴为较适宜的外植体,其愈伤组织诱导率和体细胞胚形成率最高可达96.7%和33.4%;改良SH+2,4-D 4.0 mg/L+KT 1 mg/L为最佳胚性愈伤组织诱导培养基,MSO培养基适宜三叶草胚状体的形成及植株再生。
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| 关 键 词： | 三叶草 外植体 胚性愈伤组织 胚状体 植株再生 |
| 收稿时间： | 1900-01-01; |
A study on callus induction and redifferentiation of clover |
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| WANG You-sheng,WANG Ying,LI Yang-chun.A study on callus induction and redifferentiation of clover[J].Acta Prataculturae Sinica,2009,18(2):212-215. |
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| Authors: | WANG You-sheng WANG Ying LI Yang-chun |
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| Institution: | 1.Dingxi Agricutural Technology Extensional Station, Dingxi 743000, China; 2.Dongguan Botanical Garden, Dongguan 523000, China; 3.Gansu Agricultural University, Lanzhou 730070, China |
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| Abstract: | The hypocotyls,leaf and cotyledon of four varieties of clover(Trifolum) were used to study the effect of hormone concentrations on callus induction and redifferentiation.Different varieties and explants each had different induction requirements.The hypocotyl callus induction rate of Trifolium hybridum was high(96.7%),while that of somatic embryogenesis was 33.4%.The best medium for embryogenic callus induction was modified SH+2,4-D 4.0 mg/L+KT 1 mg/L,while for embryoid redifferentiation and plant regenerati... |
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| Keywords: | clover explants embryogenic callus embryoid plant regeneration |
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