| 预处理对不结球白菜游离小孢子胚胎发生的影响 |
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| 引用本文： | 高素燕,李英,单晓政,侯喜林. 预处理对不结球白菜游离小孢子胚胎发生的影响[J]. 江苏农业学报, 2008, 24(6) |
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| 作者姓名： | 高素燕 李英 单晓政 侯喜林 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室,江苏,南京,210095;南京农业大学园艺学院,江苏,南京,210095 |
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| 基金项目： | 教育部科技重点项目(106092); 新世纪优秀人才支持计划项目(NCET-07-0439) |
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| 摘 要： | 研究了低温预处理、热激处理和高糖浓度处理对不结球白菜游离小孢子胚胎发生的影响。结果显示：对供体花蕾进行1～3d的低温预处理能显著提高出胚率，超过5d则明显降低，并且不同基因型对低温预处理的反应不同；热激处理对小孢子胚胎发生起关键作用，不同基因型所要求的最佳热激温度和时间不同；以17％的高糖浓度培养基对游离小孢子进行1～4d预处理能显著提高出胚率，随处理时间的延长效果降低，但在1～4d范围内胚诱导率均高于对照。
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| 关 键 词： | 预处理 不结球白菜 小孢子 胚胎发生 |
Effect of Pretreatment on Isolated Microspore Embryogenesis in Non-heading Chinese Cabbage |
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| GAO Su-yan,LI Ying,SHAN Xiao-zheng,HOU Xi-lin. Effect of Pretreatment on Isolated Microspore Embryogenesis in Non-heading Chinese Cabbage[J]. Jiangsu Journal of Agricultural Sciences, 2008, 24(6) |
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| Authors: | GAO Su-yan LI Ying SHAN Xiao-zheng HOU Xi-lin |
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| Abstract: | Effects of low-temperature,heat shock and higher concentration sucrose pretreatment on microspore embryogenesis in non-heading Chinese cabbage were studied.The results showed that the donor buds at low temperature pretreatment for 1-3 d could significantly improve embryo frequencies,which was decreased when the pretreatment time beyond 5 d,and different genotypes responded differently to low temperature.Heat shock treatment on microspore embryogenesis played a key role,and the suitable temperature and time ... |
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| Keywords: | pretreatment non-heading Chinese cabbage microspore embryogenesis |
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