Cu~(2+)对黄瓜发芽期发育和生理特性的影响

研究了Cu2+浓度对黄瓜发芽期发育和生理特性的影响。结果显示,低浓度Cu2+(25 mg/L)处理对黄瓜种子萌发和幼苗生长有促进作用,其种子发芽率、发芽势、发芽指数、活力指数、根长、下胚轴长、下胚轴粗度、生物量和相对含水量均高于对照及其他浓度处理;而幼苗的质膜相对透性、丙二醛含量、可溶性糖含量、脯氨酸含量及保护酶活性均在此浓度得达最低。但在高浓度Cu2+(Cu2+浓度在50~300mg/L时)处理下,随着Cu2+浓度增加,黄瓜种子萌发的各项指标开始下降,幼苗生长的各项生理指标开始上升,对黄瓜种子发芽和幼苗生长产生抑制作用,并在300mg/L时抑制作用最显著。

EFFECTS OF Cu~(2+) CONCENTRATION ON CUCUMBER SEED AND PHYSIOLOGICAL CHARACTERISTICS OF SEEDLING

Effects of different concentration Cu~(2+) on development and physiological characteristics of cucumber during seed germination were studied.The results showed that lower Cu~(2+) concentration(25mg/L) could promote the seed germination and seedling growth of cucumber,under this concentration,seed germination rate,germinating energy,germination index,vigor index,length of root,length of hypocotyls,diameter of hypocotyls and relative water content of seedlings were higher than those of control,and under other Cu~(2+) concentrations.However the plasma membrane relative permeability,MDA content,soluble sugar content,proline content,SOD and POD activities of cucumber seedling at 25mg/L Cu~(2+) were the lowest among those of all treating concentrations.But with the increasing of Cu~(2+) concentration(50～300mg/L),the physiological indicators of seed germination decreased,while physiological index of seedling had an rising tendency,which indicated that higher Cu~(2+) concentration significantly inhibited the seed germination and seedling growth,especially at 300 mg/L Cu~(2+) concentration.The results provided the reliable basis for studying the relationship between growth of crops and heavy metal Cu.