| 不同植物生长调节物质和培养基对浙江雪胆组培苗繁殖系数的影响 |
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| 引用本文： | 雷祖培,余宏傲,张书润,林瑞丰,康华靖.不同植物生长调节物质和培养基对浙江雪胆组培苗繁殖系数的影响[J].浙江林学院学报,2011,28(4). |
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| 作者姓名： | 雷祖培 余宏傲 张书润 林瑞丰 康华靖 |
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| 作者单位： | 1. 浙江乌岩岭国家级自然保护区管理局,浙江泰顺,325500
2. 温州科技职业学院园林系,浙江温州,325006 |
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| 基金项目： | 浙江省温州市科技局社会发展项目(S20080044) |
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| 摘 要： | 研究了6-苄氨基腺嘌呤(6-BA)和α-萘乙酸(NAA)2种植物生长调节物质,以及MS(Murashige and Skoog),B5(Gamborg),N6和WPM(woody plant medium)等4种培养基对浙江雪胆Hemsleya zhejiangensis组培幼苗繁殖系数的影响。结果表明:不同6-BA和NAA质量浓度对浙江雪胆组培苗繁殖系数有较大影响,其最佳质量浓度分别为0.50 mg.L-1和0.20 mg.L-1,其繁殖系数分别为10.80和5.60,且6-BA的效果显著高于NAA;而不同质量浓度的NAA和6-BA配比的最佳配方为MS+6-BA 1.00 mg.L-1+NAA 0.02 mg.L-1和MS+6-BA 1.00 mg.L-1+NAA 0.05 mg.L-1,2种配比下浙江雪胆组培苗繁殖系数分别为19.30和18.10,与单一植物生长调节物质最佳质量浓度相比,其差异也均达到了显著水平。研究还发现:浙江雪胆的组培苗在初代培养中不经生根阶段就可形成微型块茎,且该微型块茎可直接发育成新个体。
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| 关 键 词： | 植物学 浙江雪胆 组织培养 植物生长调节物质 培养基 繁殖系数 |
Hemsleya zhejiangensis seedling culture with two hormones and four types of medium |
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| LEI Zu-pei,YU Hong-ao,ZHANG Shu-run,LIN Rui-feng,KANG Hua-jing.Hemsleya zhejiangensis seedling culture with two hormones and four types of medium[J].Journal of Zhejiang Forestry College,2011,28(4). |
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| Authors: | LEI Zu-pei YU Hong-ao ZHANG Shu-run LIN Rui-feng KANG Hua-jing |
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| Institution: | LEI Zu-pei1,YU Hong-ao2,ZHANG Shu-run1,LIN Rui-feng1,KANG Hua-jing2(1.Management Office,Wuyanlin Nature Reserve of Zhejiang,Taishun 325500,Zhejiang,China,2.Department of Landscape Architecture,The Science and Technology College of Wenzhou,Wenzhou 325006,China) |
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| Abstract: | Hemsleya zhejiangensis is an endemic species in China,its population of H.zhejiangensis is declining due to the lack of seedlings and the resource volume should expanded to sustain the population exist.The tissue culture was of H.zhejiangensis maily disccused in this paper.The effects of two hormones:6-benzyladenine(6-BA) and Naphthalene acetic acid(NAA),and four types of culture media: Murashige and Skoog(MS),Gamborg(B5),N6-benzylaminopurine(N6) and woody plant medium(WPM) on the propagation of H.zhejiange... |
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| Keywords: | botany Hemsleya zhejiangensis tissue culture plant growth regulating substances culture medium propagation coefficient |
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