| 滑子蘑菌渣粗纤维素酶浸提及其酶学特性研究 |
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| 引用本文： | 马怀良,龚振杰,任荣,李大伟,郭文学.滑子蘑菌渣粗纤维素酶浸提及其酶学特性研究[J].中国食用菌,2011,30(1):48-50. |
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| 作者姓名： | 马怀良 龚振杰 任荣 李大伟 郭文学 |
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| 作者单位： | 1. 牡丹江师范学院生命科学与技术学院,黑龙江,牡丹江,157012
2. 牡丹江市绿珠果蔬技术开发有限责任公司,黑龙江,牡丹江,157000 |
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| 基金项目： | 国家科技部科技人员服务企业行动资助项目，国家"十一五"科技支撑计划 |
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| 摘 要： | 利用水浸提法浸提滑子蘑菌渣纤维素酶,选取浸提时间、液料比和温度做单因素试验,然后通过正交试验优化滑子蘑菌渣纤维素酶的浸提工艺,并对结果进行了统计分析。结果表明,浸提时间、液料比和温度对纤维素酶浸提均有极显著性影响,最佳浸提条件为温度30℃、时间1.5 h、液料比50∶1。滑子蘑菌渣羧甲基纤维素酶活力最适pH为4.4,最适温度为70℃;滤纸酶活力最适pH为6.0,最适温度为60℃。
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| 关 键 词： | 纤维素酶 浸提工艺 菌渣 滑子蘑 酶学特性 |
Extraction of Crude Cellulase from Pholiota nameko Residue and Studies on Enzymological Properties of Crude Cellulase |
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| MA Huai-liang,GONG Zhen-jie,REN Rong,LI Da-wei,GUO Wen-xue.Extraction of Crude Cellulase from Pholiota nameko Residue and Studies on Enzymological Properties of Crude Cellulase[J].Edible Fungi of China,2011,30(1):48-50. |
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| Authors: | MA Huai-liang GONG Zhen-jie REN Rong LI Da-wei GUO Wen-xue |
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| Institution: | MA Huai-liang1,GONG Zhen-jie1,REN Rong1,LI Da-wei1,GUO Wen-xue2(1.College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang Heilongjiang 157012,2.Mudanjiang Green-ball Fruits and Vegetables Technology Development Co.,Ltd.,Mudanjiang Heilongjiang 157000) |
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| Abstract: | In this study,water extract method was adopted to extract crude cellulase from Pholiota nameko residue.While liquid-material ratio and temperature were selected as single factor experiment,and then orthogonal experiments of extracting cellulase were conducted and its results were statistically analyzed.Results indicated that extraction time,extraction liquid-material ratio and extraction temperature exhibited a highly significant effect on crude cellulase from Pholiota nameko residue.It was the optimal cond... |
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| Keywords: | Cellulase Extraction Technology Mushroom Residue Pholiota nameko Enzymological Properties |
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