| 利用AFLP和ISSR标记评价蜡梅种内杂交一代群体的分离方式 |
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| 引用本文： | 陈丹维,赵凯歌,陈龙清. 利用AFLP和ISSR标记评价蜡梅种内杂交一代群体的分离方式[J]. 北京林业大学学报, 2010, 0(Z2) |
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| 作者姓名： | 陈丹维 赵凯歌 陈龙清 |
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| 作者单位： | 华中农业大学园艺林学学院园艺植物生物学教育部重点实验室; |
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| 基金项目： | 国家自然科学基金项目(30571310) |
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| 摘 要： | 以2株花部观赏形态差别较大的蜡梅‘素心’单株H4和‘红心’H29作为亲本,杂交获得64个子代个体。利用AFLP和ISSR技术对F1代群体的分离方式进行了分析,结果显示:12对AFLP引物组合和9条ISSR引物扩增获得的838条谱带在F1代呈3种分离方式:孟德尔分离、偏孟德尔分离和异常分离,分别为634(75.66%)、144(17.18%)、85(10.14%)条;在符合孟德尔分离的标记中,可用于图谱构建的分离比符合1:1和3:1的条带共有166条。研究结果表明,"双假测交模型"适用于蜡梅这种木本观花灌木,此F1代群体可用于蜡梅遗传图谱的构建。
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| 关 键 词： | 双假测交 遗传图谱 分离 |
Evaluation of segregation patterns in an intraspecific F_1 hybrid population of wintersweet (Chimonanthus praecox (L.) Link) using AFLP and ISSR markers |
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| CHEN Dan-wei,ZHAO Kai-ge,CHEN Long-qing.Key Laboratory of Horticultur al Plant Biology of Ministry of Education,College of Horticulture , Forestry Science,Huazhong Agricultural University,Wuhan,,P.R.China.. Evaluation of segregation patterns in an intraspecific F_1 hybrid population of wintersweet (Chimonanthus praecox (L.) Link) using AFLP and ISSR markers[J]. Journal of Beijing Forestry University, 2010, 0(Z2) |
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| Authors: | CHEN Dan-wei ZHAO Kai-ge CHEN Long-qing.Key Laboratory of Horticultur al Plant Biology of Ministry of Education College of Horticulture Forestry Science Huazhong Agricultural University Wuhan P.R.China. |
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| Affiliation: | CHEN Dan-wei,ZHAO Kai-ge,CHEN Long-qing.Key Laboratory of Horticultur al Plant Biology of Ministry of Education,College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan,430070,P.R.China. |
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| Abstract: | A F1 population of wintersweet(Chimonanthus praecox(L.) Link),which consisted of 64 individuals,was derived from a cross between two elite cultivars'Concolor'H4 and 'Patens'H29.The amplified fragment length polymorphism(AFLP) and inter-simple sequence repeat polymorphism(ISSR) analysis techniques were used in this study for evaluating the segregation patterns of this population.The results showed that amplification bands by AFLP and ISSR markers were grouped into three types:normal Mendelian segregation,dev... |
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| Keywords: | double pseudo-testcross genetic map segregation |
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