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紫花苜蓿苗期抗寒敏感性生理生化指标的筛选
引用本文:秘一先,鲁学思,马周文,廖斌太,郭志强,师尚礼.紫花苜蓿苗期抗寒敏感性生理生化指标的筛选[J].草原与草坪,2016(1):35-42.
作者姓名:秘一先  鲁学思  马周文  廖斌太  郭志强  师尚礼
作者单位:甘肃农业大学 草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃 兰州,730070
基金项目:农业部“牧草种质资源保护”项目(NB2130135)
摘    要:通过测定俄罗斯杂花苜蓿和3个紫花苜蓿抗寒品系苗期的叶部及茎部的生理生化指标,研究了供试材料苗期的抗寒性生理生化指标对低温的响应并筛选出紫花苜蓿苗期抗寒性敏感生理生化指标。结果表明,紫花苜蓿苗期叶部GPX、FP、APX、SOD、MDA为最敏感性指标,CAT,SS和SP为较敏感性指标;茎部SP,MDA和FP为最敏感性指标,CAT,SS和SOD为较敏感性指标;苗期叶部、茎部APX、GPX为不敏感性指标。采用最敏感性指标和较敏感性指标综合评价了供试材料的抗寒性,其抗寒能力强弱顺序为:抗寒2号抗寒3号抗寒1号俄罗斯杂花苜蓿。

关 键 词:紫花苜蓿  苗期  抗寒敏感性  生理生化指标

Screening of sensitive physiological and biochemical indexes for cold resistance of alfalfa in seedling stage
Abstract:By measuring physiological and biochemical indexes of leaves and stems of Medicago varia and 3 cold resistant strains of alfalfa in seedling stage,the response of physiological and biochemical indexes to low temperature was studied and the screening of sensitive physiological and biochemical indices in seedling was con-ducted.The results showed that GPX,FP,APX,SOD and MDA were the most sensitive indicators,CAT,SS and SP were the more sensitive indicators for alfalfa leaves;SP,MDA and FP were the most sensitive indicators, CAT,SS and SOD were the more sensitive indicators for stems,APX and GPX were not the sensitive indicators for alfalfa leaves and stems.By using comprehensive evaluation,the cold-resistance order of alfalfa was K2> K3>K1> Ru.
Keywords:alfalfa  seedling stage  cold-resistance sensibility  physiological and biochemical index
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