海南省芒果细菌性黑斑病原菌生理生化特性初步研究

为了明确海南省芒果细菌性黑斑病原菌的生理生化特性,为该病害的科学防治提供理论基础。从海南省4县市分离获得12株芒果细菌性黑斑病原菌（Xanthomonas campestris pv.mangiferaeindicae）菌株并对其进行13项生理和7项生化特性测定。结果表明,在所测试的13项生理特征中有9项耐盐性（1%～3%）、pH（5.0～8.0）、吐温80、醋酸盐、蔗糖、α-半乳糖、乳糖、麦芽糖和甘露醇]反应结果完全相同,但对果糖、葡萄糖、鼠李糖和木糖等碳源的利用却有明显差异。在7项生化试验项目中,除HNMG15产H2S试验为阴性外,其余供试菌株的氧化酶、过氧化氢酶、淀粉酶、硝酸盐还原、淀粉水解、明胶水解及产硫化氢等生化试验结果则完全一致。利用NTSYS-pc 2.10软件采用Jaccard’s相似系数和UPGMA方法对12株供试菌株的生理生化特征进行聚类分析,发现不同地理来源的芒果细菌性黑斑病原菌菌株可以聚成一类,由此可以说明海南芒果细菌性黑斑病原菌在生理生化特性方面存在一定的差异。

Preliminary Study on Physiological and Biochemical Features of the Pathogen of Mango Bacterial Black Spot in Hainan Province

In order to understand the physiological and biochemical features of Xanthomonas campestris pv.mangiferaeindicae and provide theoretical basis for scientific control of mango bacterial black spot, thirteen physiological and seven biochemical indexes of twelve Xanthomonas campestris pv. mangiferaeindicae strains from four cities（or counties） of Hainan Province were identified in this study. The results showed that nine out of thirteen physiological features were identical, including NaCl tolerance, pH tolerance, tween-80, acetate,sucrose, galactose, lactose, maltose and mannite, while the utilization of fructose, glucose, rhamnose and xylose was obviously different. Among seven tested biochemical features, oxidase, catalase, and amylase reaction,nitrate reduction, starch hydrolysis and gelatin hydrolysis were identical except H2 S. Jaccard＇s geneticsimilarity coefficients were calculated and dendrograms were constructed by the unweighted pair group method of arithmetic average（UPGMA） using the NTSYS-pc version 2.10. Different cluster analysis of physiological and biochemical tests indicated that strains from different cities or counties could share the same class, which showed that there were some diversities among Xanthomonas campestris pv. mangiferaeindicae in Hainan Province.