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新城疫、禽流感病毒同胚接种的研究
引用本文:王裕民.新城疫、禽流感病毒同胚接种的研究[J].青海畜牧兽医杂志,2009,39(4):12-13,31.
作者姓名:王裕民
作者单位:青海省互助县蔡家堡乡畜牧兽医服务中心,蔡家堡,810500
摘    要:根据新城疫病毒、禽流感病毒两种病毒均能在鸡胚中繁殖的特点,将两种病毒以最适稀释比例稀释后,分别接种于9—11日龄的鸡胚尿囊腔,同时进行两种病毒同胚培养,并利用血凝试验进行病毒效价的测定。结果表明,在同一鸡胚中两种病毒均能较好地增殖,病毒间干扰现象不明显。本试验在增强疫苗的免疫原性和进一步阐明病毒的增殖机理方面具有重要意义。

关 键 词:新城疫病毒  禽流感病毒  同胚接种

Study on Inoculation in Same Embryo for Newcastle Disease Virus and Arian Influenza Virus
WANG Yu-min.Study on Inoculation in Same Embryo for Newcastle Disease Virus and Arian Influenza Virus[J].Chinese Qinghai Journal of Animal and Veterinary Sciences,2009,39(4):12-13,31.
Authors:WANG Yu-min
Institution:Servicl Centre of Animal and Veterinary Sciencls in Caijiabao Country of Huzhu County;Qinghai Provincl;Caijiabao;810500
Abstract:According to the property of Newcastle disease virus and avian influenza virus which were able to breed in chicken embryo, the Newcastle disease virus and avian influenza virus which were diluted in the optimal proportion were respectively inoculated into the aUantoic caviry of chicken embryo at the age of 9 - 11 days and two viruses were cultured in the same embryo,The virus titers were detected by hemagglutination assay, The results indicated that the two viruses could be breeded better in the same chicken embryo, There was not the remarkable interfere phenomenon between the two viruses. The experiment results were an important significamcl in strengthening the immunogenicity of vaccine and further expounding the multiplication mechanism of virus.
Keywords:Newcastle disease virus  Avian influenza virus  Inoculation in the same embryo  
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