| 片形吸虫DNA随机扩增多态性分析 |
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| 引用本文： | 胥全彬,沈永林,A.Daugschies.片形吸虫DNA随机扩增多态性分析[J].中国兽医学报,2001,21(5):443-446. |
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| 作者姓名： | 胥全彬 沈永林 A.Daugschies |
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| 作者单位： | 1. 南京农业大学动物医学院,江苏南京,210095
2. 德国汉诺威兽医学院寄生虫研究所,汉诺威,30559 |
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| 基金项目： | 欧盟资助国际合作课题(INCO-DCIC18 CT950001) |
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| 摘 要： | 为区别从南京市江宁县采集的片形吸虫非典型形态虫体，应用随机扩增多态性DNA（RAPD）技术，对6株片形吸虫总DNA进行了扩增。结果，10条引物中有8条能产生扩增图谱，电泳图谱经聚类分析，与传统的分类结果一致，并表明来自江宁的片形吸虫既有形态典型的肝片形吸虫，也有形态不典型的大片形吸虫。
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| 关 键 词： | RAPD 片形吸虫 多态性 分类鉴别 |
| 文章编号： | 1005-4545(2001)05-0443-04 |
| 修稿时间： | 2000年9月16日 |
Detection of Difference Among Fasciola hepatica and Fasciola gigantica with RAPD |
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| XU Quan bin ,SHEN Yong lin ,A.Daugschies.Detection of Difference Among Fasciola hepatica and Fasciola gigantica with RAPD[J].Chinese Journal of Veterinary Science,2001,21(5):443-446. |
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| Authors: | XU Quan bin SHEN Yong lin ADaugschies |
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| Institution: | XU Quan bin 1,SHEN Yong lin 1*,A.Daugschies 2 |
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| Abstract: | Ten primers are applied to detect the difference among Fasciola hepatica and Fasciola gigantica from China,England and German.The amplification result shows that eight out of ten primers can produce DNA fingerprint.After cluster analysis with SPSS software,the RAPD results coincide with the results by morphology.The assay also substantiates that the Fasciola from Jiangning county in China consist of both the Fasciola hepatica with identical morphology and Fasciola gigantica without identical morphology. |
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| Keywords: | RAPD Fasciola polymophism identification |
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