纤维蛋白胶复合物促进犬骨折愈合的临床学观察

为了探索纤维蛋白胶(FG)联合人骨形态发生蛋白(rhBMP-2)、碱性成纤维细胞生长因子(bFGF)和妥布霉素的复合物对犬骨折愈合的影响,建立了犬胫骨骨折模型,将FG复合物应用于实验组和空白对照组的胫骨骨折处,在术后2、4、8、12、16周,测定骨折处周围软组织充血水肿消除时间、利用X射线影像学评价骨折愈合程度、测定骨折愈合部位的骨密度(BMD)及骨密度比率、骨痂体积,测定胫骨生物力学强度并进行统计和分析。实验组和对照组比较:术部红、肿、热消失时间显著缩短,术后各时间点的X射线影像学评分更高,术后各时间点骨痂更大,术后16周术部骨骼能承受的最大载荷和最大位移显著增大。结果表明FG联合rhBMP-2、bFGF与妥布霉素应用于骨折部位,可促进骨折愈合过程中软组织的生成,缩短骨折愈合的时间,提高骨生物力学强度。

ClinicalSObservationSonSFibrinSGlueSCompoundSonSPromotingSFractureSHealingSinSDogs

To explore the influence of fibrin glue(FG)combined human bone morphogenetic protein(rhBMP-2),basic fibroblast growth factor and tobramycin complex on the healing of dog fracture.Establishing dog tibia fracture model,applying the FG complex to the place of tibia fracture in the experimental and blank control group,in the 2nd,4th,12th and 16th week after the surgery,measuring the time for elimination of congestion and edema in the soft tissue around the fracture,evaluating the fracture healing extent by using X ray imaging,measuring the bone mineral density(BMD)and bone mineral density rate,callus volume,measuring tibia biomechanical properties and make statistics and analysis.Comparison between the experimental group and contrast group:The time for disappearing of red,swollen,heat at the place of the surgery is significantly reduced,the scoring of X ray imaging is higher at each time point after the surgery,and the callus at each time point after the surgery is larger,in the 16th week after the surgery,the maximum load and displacement for the bone at the place of the surgery is significantly enhanced.By applying FG,rhBMP-2、bFGF and tobramycin in the place of fracture,it could promote the generation of soft tissue in the fracture healing process,reduce the fracture healing time and im0prve he bone biomechanical properties.