花脸香蘑菌丝体发酵条件优化研究

目的]优化花脸香蘑菌丝体发酵条件。方法]研究花脸香蘑菌丝体发酵中碳源、氮源、pH、时间对菌丝干重的影响。结果]培养条件中对花脸香蘑菌丝体生长影响的顺序为:碳源>时间>pH>氮源。通过正交优化试验确定了花脸香蘑菌丝体液体最佳发酵条件为:玉米粉3%、酵母膏0.2%、pH值6.0、培养时间8 d。结论]该研究结果为开发利用花脸香蘑生物资源提供了理论参考和试验依据。

Optimization of Fermentation Condition of Lepista sordida Mycelium

Objective] This study aimed to optimize the fermentation condition of Lepista sordida mycelium.Method] The influences of carbon sources,nitrogen sources,pH and time on the dry weight of mycelium in Lepista sordida fermentation were investigated.Result] The descending order of the influences on Lepista sordida mycelium growth was: carbon sources>time>pH>nitrogen sources.Based on orthogonal test,the optimal fermentation condition of Lepista sordida mycelium was determined as: fermentation medium of 3% corn starch and 0.2% yeast extract,pH of 6.0,and incubation time of 8 d.Conclusion] This study provides theoretical reference and experimental basis for the development and utilization of Lepista sordida biological resources.