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| 人工养殖中华鲟鱼皮中胶原蛋白提取工艺研究 | |
| 引用本文： | 冯伟伟,邹艳敏,茆广华,赵婷,邹烨,郑大恒,仰榴青,吴向阳.人工养殖中华鲟鱼皮中胶原蛋白提取工艺研究[J].安徽农业科学,2012,40(25):12653+12673. |
| 作者姓名： | 冯伟伟 邹艳敏 茆广华 赵婷 邹烨 郑大恒 仰榴青 吴向阳 |
| 作者单位： | 1. 江苏大学药学院,江苏镇江,212013 2. 江苏大学食品与生物工程学院,江苏镇江,212013 3. 江苏大学化学化工学院,江苏镇江,212013 4. 江苏大学环境学院,江苏镇江,212013 |
| 摘 要： | 目的]研究确定人工养殖中华鲟鱼皮中胶原蛋白的酶法提取工艺。方法]以胶原蛋白得率为指标,采用单因素试验,考察各因素对人工养殖中华鲟鱼皮中胶原蛋白得率的影响。结果]胃蛋白酶为最适宜提取酶,人工养殖中华鲟鱼鱼皮中胶原蛋白提取的最佳条件为料液比1∶10 g/ml,加酶量2.0%,提取时间6 h,胶原蛋白的得率为82.7%,含量为49.9%。结论]该提取工艺稳定,提取率高,适用于人工养殖中华鲟鱼鱼皮中胶原蛋白的提取。 |
| 关 键 词： | 鲟鱼 胶原蛋白 酶法提取 人工养殖 单因素试验 |
Study on the Extraction Technology of Collagen from Artificially Cultured Acipenser sinensis Skin |
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| Institution: | FENG Wei-wei et al(School of Pharmacy,Jiangsu University,Zhenjiang,Jiangsu 212013) |
| Abstract: | Objective] To research the extraction technology of collagen from skin of artificially cultured Acipenser sinensis.Method] Based on the index of the yield of pepsin-soluble collagens,single factor experiments were conducted to observe the influence of each factor on the yield of collagens.Result] Pepsin is the most suitable enzyme for the extraction of collagen.The optimum conditions of extraction were obtained as follows: the solid-liquid ratio was 1∶ 10(g/ml),the dosage of pepsin was 2.0% and the extraction time was 6 h.The yield of collagen was 82.7% and the content of collagen was 49.9%.Conclusion] The extraction technology is stable,and has high yield.It can be used for the extraction of collagens. |
| Keywords: | Sturgeon Collagen Enzymatic extraction Artifical culture Single factor experiment |
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