金边瑞香花粉活力和柱头可授性研究

目的]研究金边瑞香花粉活力和柱头可授性。方法]采用I-KI法、TTC法和固体培养基法测定金边瑞香花粉的活力,并用联苯胺-过氧化氢法测定柱头的可授性。结果]金边瑞香从初花期至盛花期,I-KI法未对花粉内部染色,使用TTC法花粉未变红色,离体培养法未见有花粉管萌发,均表明花粉不具生活力。柱头表面有少量气泡产生的,且柱头变色,表明金边瑞香柱头可授性较低。结论]金边瑞香花粉无活力,柱头可授性低。

Pollen Viability and Stigma Receptivity of Daphne odora var.marginata

Objective] To study the pollen viability and stigma receptivity of Daphne odora var.marginata.Method] The pollen viability was detected by the methods of iodine-iodide Kalium(I-KI),triphenyltrazolium chloride(TTC) and solid medium.The stigma receptivity was estimated by benzidine-H2O2 method.Result] The pollen was not stained by I-KI method,and did not become red by TTC method,and there was no germinated pollen tube by the in vitro culture method,indicating that the pollen viability was zero during the whole flowering period of Daphne odora var.marginata.Few bubbles appeared on the stigma surface and the stigma color changed,which meant that stigma receptivity was very low.Conclusion] Daphne odora var.marginata has no pollen viability and low stigma receptivity.