| 酶法辅助提取南板蓝叶中靛玉红的工艺研究 |
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| 引用本文： | 黄凤婷,马永良,林春颖,熊清平.酶法辅助提取南板蓝叶中靛玉红的工艺研究[J].安徽农业科学,2012,40(26):12866-12867,12879. |
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| 作者姓名： | 黄凤婷 马永良 林春颖 熊清平 |
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| 作者单位： | 广州中医药大学第三附属医院芳村分院,广东广州,510360;中山大学孙逸仙纪念医院,广东广州,510120;淮阴工学院,江苏淮安,223003 |
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| 基金项目： | 广州市卫生局中医科研课题资助项目(2009-A-50);广东省中医药局建设中医药强省科研课题项目(2009303) |
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| 摘 要： | 目的]研究酶法提取关键参数对南板蓝叶中靛玉红提取工艺的影响。方法]采用HPLC测定靛玉红的含量,并考察酶用量、酶解pH值和酶解时间对提取率的影响,并优选出了最佳工艺条件。结果]南板蓝叶中靛玉红酶法辅助提取的最佳工艺条件为:酶用量25 U/g,酶解pH值4.8,酶解时间6 h;在此条件下,靛玉红的Z值为7.02%。结论]酶法辅助提取法适用于南板蓝叶中靛玉红的提取。
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| 关 键 词： | 酶法辅助提取 靛玉红(Indirubin) 南板蓝根(Baphicacanthus cusia(Nees)Bremek) |
Study on Enzyme-assisted Extraction of Indirubin from Baphicacanthus cusia(Nees) Bremek Leaves |
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| Institution: | HUANG Feng-ting et al(Fangcun Branch of the 3rd Affiliated hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong 510360) |
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| Abstract: | Objective] To observe the key parameters of enzymatic extraction that impact extraction of indirubin from Baphicacanthus cusia(Nees) Bremek leaves.Method] The content of indirubin was determined using HPLC.Effects of enzyme dosage,hydrolysis pH and hydrolysis time on extraction rate of indirubin were investigated with the aim of optimizing its extraction technology.Result] The optimal enzyme-assisted extraction conditions of indirubin from B.cusia leaves were the combination of enzyme dosage 25 U/g,hydrolysis pH 4.8 and hydrolysis time 6 h.Under the above conditions,the Z value of indirubin was 7.02%.Conclusion] Enzyme-assisted extraction method is suitable for extraction of indirubin from B.cusia leaves. |
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| Keywords: | Enzyme-assisted extraction Indirubin Baphicacanthus cusia(Nees) Bremek |
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