GA_3和CEPA对香水百合开花期光合生理和抗氧化酶活性的影响及其花期响应研究

以香水百合为材料,采用盆栽试验,研究了在不同浓度赤霉素(GA3)、乙烯利(CEPA)的处理影响下,香水百合的花期(现蕾期、初花期、盛花期和末花期)变化、光合生理和抗氧化酶活性的变化及其花期响应。结果表明,1)在香水百合开花期,GA3和CEPA处理均不同程度地提高了香水百合株高、茎粗、蕾长和花径,并提前了初花期,延长了开花时间。GA3与株高、茎粗和蕾长呈显著正相关,对开花前中期影响显著,而CEPA与茎粗、蕾长和花径呈显著正相关,与末花天数呈显著负相关;2)GA3和CEPA处理提高了各时期光合色素含量,并有效缓解了末花期叶绿素的下降趋势,延长了光合作用时间;3)GA3和CEPA处理显著提高了香水百合开花期Pn、Gs、Tr和Ls,而Ci和LUE与其他参数呈负相关关系,分析表明,GA3和CEPA处理增强了花期叶片细胞的活性,提高了叶片的光合作用强度;4)GA3和CEPA处理的百合叶片MDA含量在开花各时期显著低于对照,抗氧化酶SOD、CAT活性上升,同时,处理减缓了开花中后期SOD下降趋势,有效缓解了衰老进程,达到延长香水百合花期的目的。综合分析表明,GA3和CEPA提高了香水百合开花各时期的光合特性,并增强了抗氧化酶活性,进而对香水百合开花品质和开花花期有显著影响。其中,GA3200mg/L+CEPA 30mg/L协同处理对提前花期和延长开花持续天数的调节效果最佳,其次为单水平GA3200mg/L处理和单水平CEPA 60mg/L处理,高浓度GA3200mg/L+CEPA 60mg/L和GA3300mg/L+CEPA 30mg/L的调节作用下降。

Effects of GA3 and CEPA on photosynthetic characteristics and antioxidant enzymes in the flowering phase and the flowering response of Lilium casa blanca

The process changes of flowering phase (the squaring stage,the early flowering stage,blooming stage,ending flowering)and the changes of photosynthetic characteristics,antioxidant enzymes,the flowering response of Lilium casa blanca were studied using the bulbs of L.casa blanca as pot experimental material un-der the coordination treatment of gibberellin (GA3 )and ethrel (CEPA).The results showed that,1 )It in-creased the plant height,thick stems,bud length and flower diameter,advanced the blooming stage and ex-tended the days of blossom of L.casa blanca under the treatment of GA3 and CEPA during the flowering
phase.There were significantly positive correlation between GA3 and plant height,stem diameter and bud length,while there were a significantly positive correlation between CEPA and stem diameter,bud length and flower diameter.GA3 made a significant influence in earlier and middle flowering in L.casa blanca .However, CEPA made a significantly negative correlation with ending flowering;2)The treatment of GA3 and CEPA both advanced the photosynthetic pigment in leaves of L.casa blanca ,and alleviated the downward trend of chlorophyll at the ending flowering time effectively,also it prolonged the photosynthesis time in leaves;3)The treatment of GA3 and CEPA both significantly promoted photosynthetic rate (P n ),conductance to H2 O (G s ), transpiration rate (T r )and stomatal limitation value (L s ),But there were a significantly negative correlation between intercellular CO2 concentration (C i ),transient energy utilization efficiency (LUE)and others.Analy-sis showed that,the treatment of GA3 and CEPA enhanced the activity of leaf cells,increased the intensity of photosynthesis in leaf;4)MDA content in leaves is lower than CK significantly under the treatment of GA3 and CEPA during the flowering phase,and the activity of SOD and CAT increased.At the same time,the treat-ment of GA3 and CEPA slowed down the downward trend of SOD activity in the middle and ending flowering time,relieved the ageing process of L.casa blanca ,and it reached the purpose of extending the time of blos-som of L.casa blanca .Comprehensive analysis shows that,the treatment of GA3 and CEPA advanced the pho-tosynthetic characteristics and antioxidant enzymes of L.casa blanca ,It made a significant influence in the blossom quality and flowering phase.Among them,the coordination treatment GA3 200 mg/L+CEPA 30 mg/L is the best in regulation on advancing flowering time and extending the days of blossom in L.casa blanca , followed by the single treatment CEPA 60 mg/L and GA3 200 mg/L.However,the adjust action decreased in high concentrations coordination treatment GA3 200 mg/L+CEPA 60 mg/L and GA3 300 mg/L+CEPA 30 mg/L.