Vc对猪体细胞克隆胚胎体外及体内发育的影响 |
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引用本文: | 周荣,罗绿花,麦然标,余婉娴,贺晓燕,纪红美,石俊松,王青来,蔡更元,吴珍芳.Vc对猪体细胞克隆胚胎体外及体内发育的影响[J].安徽农业科学,2016,44(23). |
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作者姓名: | 周荣 罗绿花 麦然标 余婉娴 贺晓燕 纪红美 石俊松 王青来 蔡更元 吴珍芳 |
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作者单位: | 广东温氏食品集团股份有限公司国家生猪种业工程技术研究中心,广东新兴,527439;广东温氏食品集团股份有限公司国家生猪种业工程技术研究中心,广东新兴527439;华南农业大学动物科学学院,广东广州510642 |
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基金项目: | 国家转基因生物新品种培育项目(2016ZX08006002;2016ZX08006003)。 |
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摘 要: | 目的]提高克隆猪的大批量生产效率,优化体外与体内培养体系。方法]将所获得的猪体细胞克隆胚胎用含不同Vc浓度的培养液培养,比较体外囊胚发育力和囊胚细胞数,筛选出合适的培养浓度,然后用此浓度培养后的胚胎进行体内手术移植,统计克隆猪的分娩率和产仔情况。结果]克隆胚胎在激活后用40μg/m L Vc处理22 h,体外培养未显著增加囊胚率,但显著提高了囊胚细胞数。体内移植结果显示,添加Vc未提高妊娠率,但增加了窝均总仔数,克隆效率差异显著。结论]Vc处理有利于增强体细胞克隆胚的体内和体外发育能力。
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关 键 词: | Vc 猪 克隆胚胎 发育 |
Effects of Vitamin C on the Development of Porcine Somatic Cloned Embryos in vitro and in vivo |
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Abstract: | Objective] To improve the ability to generate cloned pigs and to optimize the culture system in vitro and in vivo.Method] The por-cine SCNT embryos were cultured in the medium with different vitamin C concentrations .Then the blastocyst formation rate and cell number per blastocyst were compared.Proper concentration was screened,which was used to treat cloned embryos.After surgery transfer,farrowing rate and litter size were calculated.Result] After stimulation,cloned embryos were treated by 40μg/mL Vc for 22 h;culture in vitro did not significantly enhance the blastocyst rate,but the blastocyst cell number was significantly increased.Results of in vivo test showed that adding Vc did not signifi-cantly enhance the pregnancy rate,but increased the average litter size.There were significant differences in the cloning efficiency.Conclusion] Vc treatment is beneficial to the development of porcine SCNT embryos both in vivo and in vitro. |
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Keywords: | Vitamin C Porcine Cloned embryos Development |
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