| 苯酚硫酸法与蒽酮硫酸法测定托盘根多糖含量的比较 |
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| 引用本文： | 郑旭伟,黄占波,楚冬海,王晓宇,宁雪,黄丽莉.苯酚硫酸法与蒽酮硫酸法测定托盘根多糖含量的比较[J].安徽农业科学,2016,44(23):119-121. |
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| 作者姓名： | 郑旭伟 黄占波 楚冬海 王晓宇 宁雪 黄丽莉 |
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| 作者单位： | 辽宁科技学院生物医药与化学工程学院,辽宁本溪,117004;辽宁科技学院生物医药与化学工程学院,辽宁本溪,117004;辽宁科技学院生物医药与化学工程学院,辽宁本溪,117004;辽宁科技学院生物医药与化学工程学院,辽宁本溪,117004;辽宁科技学院生物医药与化学工程学院,辽宁本溪,117004;辽宁科技学院生物医药与化学工程学院,辽宁本溪,117004 |
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| 基金项目： | 辽宁科技学院博士科研启动基金(1406B05)；辽宁省大学生创新训练项目(201611430024)。 |
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| 摘 要： | 目的]采用苯酚硫酸法和蒽酮硫酸法测定托盘根多糖含量。方法]用精制托盘根多糖测得对葡萄糖的换算因子,通过线性关系、精密度、重现性、稳定性及加样回收率等指标进行综合评价。结果]葡萄糖在5~40μg/m L范围内浓度与吸光度呈良好的线性关系,重复性试验相对标准偏差1.7%,加样回收率≥97%,苯酚硫酸法和蒽酮硫酸法测定的多糖含量分别为3.78%和4.02%。结论]苯酚硫酸法和蒽酮硫酸法均准确可靠,适合于托盘根多糖的测定。
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| 关 键 词： | 托盘根 多糖 苯酚-硫酸法 蒽酮-硫酸法 |
Comparison of Phenol-sulfuric Acid and Anthrone-sulfuric Acid Methods for Determination of Polysaccharide in Rubus crataegiflolius Bunge |
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| Abstract: | Objective] The phenol-sulfuric acid method and anthrone-sulfuric acid method were compared on determination of the polysac-charide content in Rubus crataegiflolius Bunge..Method] The conversion factor was measured with refined polysaccharide for glucose, and comprehensive evaluation was carried out by linear relationship, precision, reproducibility, stability and recovery rate. Result] The results showed that the linear relationship between glucose concentration and absorbance was good in the range of 5-40 μg/mL, and the average recov-ery rate was equal to or higher than 97% with good reproducibility (RSD=1.7%), the content of polysaccharide determined by phenol-sulfu-ric acid method and anthrone-sulfuric acid method were 3.78% and 4.02% . Conclusion] Phenol-sulfuric acid and anthrone-sulfuric acid methods are accurate and reliable, and suitable for determination of polysaccharide in R.crataegiflolius. |
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| Keywords: | Rubus crataegiflolius Bunge Polysaccharides Phenol-sulfuric acid method Anthrone-sulfuric acid method |
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