| 木奶果醇提物的抗氧化活性及其对Aβ25-35致PC12细胞损伤的神经保护作用 |
| |
| 引用本文： | 郑晓燕,盛占武,谢学历,阙燕鹏,谭琳,艾斌凌,郑丽丽.木奶果醇提物的抗氧化活性及其对Aβ25-35致PC12细胞损伤的神经保护作用[J].热带作物学报,2018,39(1):174-181. |
| |
| 作者姓名： | 郑晓燕 盛占武 谢学历 阙燕鹏 谭琳 艾斌凌 郑丽丽 |
| |
| 作者单位： | 1. 中国热带农业科学院海口实验站
2海南省香蕉遗传育种重点实验室
3 海南医学院海南省安评中心
4海南大学农学院 |
| |
| 摘 要： | 为了探明木奶果不同部位的总酚含量和抗氧化活性及其对Aβ25-35致PC12细胞损伤的神经保护作用的差异,以期为木奶果高价值产品开发提供理论依据。以木奶果果皮、果肉、果核3个部位醇提物为研究对象,采用Folin-Ciocalteu法、DPPH、ABTS、羟基自由基清除能力测定体外抗氧化活性,并采用Aβ25-35诱导PC12细胞成阿尔兹海默病细胞模型测定醇提物对不同浓度Aβ25-35所致PC12细胞损伤的神经保护作用。结果显示:木奶果果皮、果肉、果核3个部位的总酚含量分别为(101.03±5.99)、(16.03±1.13)、(51.27±4.02)mg GAE/g干物质;抗氧化活性与样品浓度存在剂量关系,其中果皮的抗氧化活性最强,果核次之,果肉最差;果皮醇提物对Aβ25-35所致PC12细胞损伤的神经保护作用最佳,当木奶果果皮醇提物浓度为1 mg/m L时,能将Aβ25-35诱导的PC12细胞致死率从(41.6±1.36)%降为(11.95±1.98)%;PC12细胞的凋亡率从(84.69±5.78)%降至(25.26±3.18)%。可见,木奶果醇提物对Aβ25-35所致PC12细胞氧化损伤具有很好的保护作用。
|
| 关 键 词： | 木奶果 醇提物 抗氧化 神经保护 |
Antioxidant Activity of Ethanol Extracts of Baccaurea Fruit and Its Neuroprotective Effect on PC12 Cells Damage Induced by Aβ25-35 |
| |
| ZHENG Xiaoyan,SHENG Zhanwu,XIE Xueli,QUE Yanpeng,TAN Lin,AI Binling,ZHENG Lili.Antioxidant Activity of Ethanol Extracts of Baccaurea Fruit and Its Neuroprotective Effect on PC12 Cells Damage Induced by Aβ25-35[J].Chinese Journal of Tropical Crops,2018,39(1):174-181. |
| |
| Authors: | ZHENG Xiaoyan SHENG Zhanwu XIE Xueli QUE Yanpeng TAN Lin AI Binling ZHENG Lili |
| |
| Institution: | 1 Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences
2 Hainan Key Laboratory of Banana Genetic Improvement, Haikou, Hainan 570102, China;
3Hainan Medical University / Hainan Provincial Drug Safe Evaluation Research Center, Haikou, Hainan 571199, China;
4College of Agronomy, Hainan University, Haikou, Hainan 570228, China |
| |
| Abstract: | The total phenolic contents, antioxidant activity and the neuroprotective effect on PC12 cells damage induced by Aβ25 -35 of different parts of Baccaurea fruit ( BF) were investigated to provide theoretical basis for developing high value products of BF. In this study, the ethonal extracts of different parts of BF ( peel, pulp, kern) were subjected to the following assay. The total phenolic contents of the alcohol extracts were determined by Folin- Ciocalteu method, and the antioxidant activity of the ethonal extracts was evaluated by DPPH, ABTS and hydroxyl system. The neuroprotective effect was investigated by using Aβ25-35-induced damage in PC12 cells. The cell viability of PC12 cells was detected by CCK -8 assay and the apoptosis was detected by TUNEL reaction kit. The total phenolic contents of the ethonal extracts of the three parts were 101.03±5.99, 16.03±1.13, 51.27±4.02 mg gallic acid equivalents ( GAE) per 1 g dried weight respectively. The antioxidant activity increased in a concentration dependent manner. The highest antioxidant activity and neuroprotection were observed in the peel. By adding 1 mg/mL of peel extracts, the lethality rate of PC12 cell decreased from (41.6+1.36)% to (11.95+1.98)%, and the apoptosis rate of PC12 cell decreased from ( 84.69 +5.78)% to ( 25.26 +3.18)% . In conclusion, the alcohol extracts of the BF has good neuroprotective effect on PC12 cells damage induced by Aβ25-35. |
| |
| Keywords: | baccaurea ethanol extracts antioxidant neuroprotection |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
