猪4号和7号染色体部分微卫星位点的遗传图谱构建

摘要：应用3头英系大白公猪(Sus scrofa )与7头梅山母猪(S. scrofa )杂交建立了参考家系,采集了140头F2代个体的血样,从USDA-MARC公布的4和7号染色体上分别选择7和8个微卫星位点,对这些微卫星位点检测了每个个体的基因型,应用CRIMAP软件,构建了猪4号和7号染色体的连锁图谱。在这－群体中,15个微卫星位点的平均等位基因数为3.6个,平均杂合度和平均多态信息含量分别为0.531和0.463,平均有信息减数分裂数为235。研究所构建的4号染色体两性平均连锁图谱全长为178.5 cM,位点间的平均间距为29.9 cM,公、母猪连锁图谱全长分别为172.2 cM和197.7 cM;7号染色体两性平均连锁图谱全长为179.1 cM,平均间隔为24.9 cM,公、母猪连锁图谱全长分别为167.7 cM和195.7 cM。连锁图谱的构建为以后的QTL定位奠定了基础。

Genetic Linkage Map Construction of Some Microsatellites on Pig Chromosome 4 and 7

Abstract: A reference family was established by using three Large White boars (Sus scrofa )and seven Meishan dams(S.scrofa ). Blood samples of 140 F2 individuals were collected. Each sample was amplified by using primers of 7 microsatellites loci on chromosome 4 and 8 microsatellites loci on chromosome 7, and genotypes were identified. The linkage map of chromsome 4 and 7 was constructed by using CRIMAP software. In this population, average allele number was 3.6, average heterozygosity and polymorphic information content 0.531 and 0.463, average information meiosis 235. The genetic length of sex-average, female and male maps were 178.5, 197.7 and 172.2 cM for chromosome 4, and 179.1, 195.7 and 167.7 cM for chromosome 7 respectively. The average marker interval of chromosome 4 and 7 was 29.9 and 24.9 cM, respectively. Construction of linkage map will make the foundation for QTL mapping afterwards.