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感染甘薯小象甲白僵菌菌株的初步筛选
引用本文:曹伟平,宋健,王金耀,冯书亮,杜立新.感染甘薯小象甲白僵菌菌株的初步筛选[J].河北农业科学,2011,15(11):45-47.
作者姓名:曹伟平  宋健  王金耀  冯书亮  杜立新
作者单位:河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000
基金项目:河北省财政项目(F12C10034);国家“948”项目(2011-G4);国家“863”项目(2011AA10A201);国家“863”项目(2011AA10A203)
摘    要:甘薯小象甲是甘薯的主要害虫之一。为筛选对小象甲的优良生防菌株,对分离自不同寄主的8株白僵菌进行了产孢量、孢子萌发率等生物学指标研究;采用浸渍法,进行了白僵菌对甘薯小象甲成虫侵染能力的初步筛选。结果表明:白僵菌菌株Gxj-10和Xwy-1感染甘薯小象甲成虫15 d的校正死亡率分别为82.1%和60.7%,表现出较强的致病力,且在培养过程中产孢量较高。2株菌株在防治甘薯小象甲方面具有较强的研究与应用价值。

关 键 词:白僵菌  甘薯小象甲  菌株筛选  致病力  产孢量  萌发率

Screening of Beauveria bassiana Strains against Cylas formicarius
CAO Wei-ping , SONG Jian , WANG Jin-yao , FENG Shu-liang , DU Li-xin.Screening of Beauveria bassiana Strains against Cylas formicarius[J].Journal of Hebei Agricultural Sciences,2011,15(11):45-47.
Authors:CAO Wei-ping  SONG Jian  WANG Jin-yao  FENG Shu-liang  DU Li-xin
Institution:(Institute of Plant Protection,Hebei Academy of Agriculture and Forestry Sciences,IPM Center of Hebei Province,Baoding 071000,China)
Abstract:Cylas formicarius was the major pest on sweet potato. In order to screen available biocontrol strains for controlling sweet potato weevil, eight Beauveria bassiana strains isolated from different hosts were tested for the sporogenous ability and spore germination rate. The virulence of eight isolates to C. formicarius adult was tested by cuticle infection in laboratory. The results indicated that the strains Gxj-10 and Xwy-1 showed high pathogenicity on C.formicarius adult with the corrected mortality of 82. 1% and 60. 7%, respectively after 15 d infection. Strain Gxj-10 may serve as a potential source of biocontrol agents for C. formicarius.
Keywords:Beauveria bassiana  Cylas formicarius  Strain screening  Pathogenicity  Spore yield  Spore germination rate
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