| 磺胺嘧啶多克隆抗体的制备 |
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| 引用本文： | 杨继飞,职爱民,刘庆堂,邓瑞广,柴书军,张改平.磺胺嘧啶多克隆抗体的制备[J].河南农业科学,2009(5). |
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| 作者姓名： | 杨继飞 职爱民 刘庆堂 邓瑞广 柴书军 张改平 |
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| 作者单位： | 河南省农业科学院河南省动物免疫学重点实验室,河南,郑州,450002 |
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| 基金项目： | "十一五"国家科技支撑汁划 |
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| 摘 要： | 为制备磺胺嘧啶(sulfadiazine,SD)多克隆抗体(pAb),用重氮化方法将SD偶联于载体蛋白BSA和OVA上,合成免疫抗原BSA-SD和包被抗原OVA-SD。用紫外扫描、SDS-PAGE凝胶电泳试验检测,结果表明偶联成功。用BSA-SD免疫新西兰大白兔,间接ELISA测定多克隆抗体效价。阻断ELISA结果显示:SD单克隆抗体(pAb)的IC50达19.19ng/mL,和其他磺胺类药物交叉反应小于等于2.7%。结果表明,获得了高效价和特异的pAb。
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| 关 键 词： | 磺胺嘧啶 多克隆抗体 制备 |
Preparation of the Polyclonal Antibodies against Sulfadiazine |
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| YANG Ji-fei,ZHI Ai-min,LIU Qing-tang,DENG Rui-guang,CHAI Shu-jun,ZHANG Gai-ping.Preparation of the Polyclonal Antibodies against Sulfadiazine[J].Journal of Henan Agricultural Sciences,2009(5). |
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| Authors: | YANG Ji-fei ZHI Ai-min LIU Qing-tang DENG Rui-guang CHAI Shu-jun ZHANG Gai-ping |
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| Institution: | Henan Provincial Key Laboratory of Animal Immunology;Henan Academy of Agricultural Sciences;Zhengzhou 450002;China |
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| Abstract: | To prepare the polyclonal antibodies aginst sulfadiazine,the artificial immunizing antigen BSA-SD and coating antigen OVA-SD obtained by diazotization,and further identified by ultraviolet(UV) and SDS-PAGE.New Zealand white rabbits were immunized with BSA-SD.The titres of polyclonal antibodies were detected by indirect ELISA and blocking ELISA.The results showed that the IC50 reached to 19.19 ng/mL and the cross reaction with other sulfa drugs was less than or equal to 2.7%.This indicated that the SD polycl... |
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| Keywords: | Sulfadiazine Polyclonal antibody Preparation |
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