白边大叶蝉线粒体基因组测序与分析

采用引物步移法测得白边大叶蝉Kolla paulula(Walker)线粒体基因组90%左右的序列,并分析了该叶蝉的线粒体基因组特征。基于23个物种(半翅目)的蛋白编码基因序列,以最大似然法和贝叶斯法构建了头喙亚目系统发育树。已测得序列长度为13 579 bp(AT:73.33%),其中包含了13个蛋白编码基因、21个t RNA基因和1个r RNA基因。除了ND5基因使用GTT作为起始密码子外,其他所有蛋白编码基因均使用ATN作为起始密码子;除了CO II使用不完整的T作为终止密码子,其他所有蛋白编码基因均使用TAA或TAG作为终止密码子。21个t RNA基因中,除了t RNASer(AGN)缺失1个稳定的茎环结构外,其他所有t RNA基因均能形成典型的三叶草二级结构。系统发育分析结果表明,进化树明显可以被分为沫蝉总科+(角蝉总科+蜡蝉总科);白边大叶蝉属于角蝉总科的叶蝉科,白边大叶蝉线粒体基因组特征与其他叶蝉科昆虫相同。

Sequencing and analysis of the mitochondrial genome of Kolla paulula (Walker) (Hemiptera: Cicadellidae)

About 90％ of the mitochondrial genome of Kollapaulula (Walker) was sequenced using the primer extraction step method and the mitochondrial genomic characteristics was analyzed in this study.We constructed a phylogenetic tree from 23 species (Hemiptera) based on the nucleotide sequence of 13 mitochondrial protein-coding genes using Bayesian inference and Maximum likelihood.The sequence length was 13579 bp (AT:73.33％),includ ing 13 protein coding genes,21 tRNA genes and 1 rRNA gene.All protein coding genes used ATN as initiation codon except ND5 that used GTT as initiation codon.All protein coding genes used TAA or TAG as stop codon except CO Ⅱ that used incomplete T as stop codon.All of the tRNA genes formed a typical clover secondary structure except tRNASer(AGN) that was lack of a stable stem-loop structure among the 21 tRNA genes.The phylogenetic analysis results showed that the phylogenetic tree could be divided into Cercopoidea+(Membracoidea+ Fulgoroidea).The mitochondrial genome of Kolla paulula (Membracoidea:Cicadellidae) had the same characteristics as other Cicadellidae.