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农抗702抗真菌活性的测定
引用本文:周云,张智平,涂晓嵘,涂国全.农抗702抗真菌活性的测定[J].江西农业大学学报,2009,31(6).
作者姓名:周云  张智平  涂晓嵘  涂国全
作者单位:1. 江西农业大学,作物生理生态与遗传育种教育部重点实验室,农业部双季稻生理生态与栽培重点开放实验室,江西省作物生理生态与遗传育种重点实验室,南昌市发酵应用技术重点实验室,江西,南昌,330045
2. 江西农业大学,生物科学与工程学院,江西,南昌,330045
基金项目:国家科技支撑计划项目,国家自然科学基金项目,江西省自然科学基金项目 
摘    要:探索农抗702的抗真菌活性.通过以农抗702为供试材料,霉菌、酵母菌以及14种植物病原真菌为供试菌,采用菌丝生长速率法和菌丝干重法测定农抗702对各种供试菌的抑制作用.结果表明农抗702对黑曲霉、青霉、毛霉、根霉、木霉、啤酒酵母、产朊假丝酵母、稻纹枯病、稻瘟病和稻曲病的最低抑菌浓度(mg/L)分别为3.3、6.5、6.5、3.3、3.3、6.5、3.3、23.8、59.64、475.4.农抗702对供试植物病原真菌的EC_(50)(mg/L)和EC_(90)(mg/L)依次是:油菜菌核病菌为0.23和1.95,葡萄炭疽病菌为0.27和2.91,车前草穗枯病菌为0.33和8.58,烟草赤星病菌为0.89和12.02,车前草菌核病菌为1.23和4.02,小麦赤霉病菌为1.40和7.38,棉花黄萎病菌为1.53和5.07,富贵竹炭疽病菌为1.73和5.61,水稻纹枯病菌为1.89和7.03,紫荆炭疽病菌为3.21和14.17,辣椒根腐病菌为3.40和20.78,交链孢病菌为4.13和22.01,稻瘟病菌为12.51和29.36,稻曲病菌为59.42和201.80.研究表明农抗702具有较强的抗真菌活性,为农抗702的应用提供有益的试验证据.

关 键 词:链霉菌702  农抗702  抗真菌活性  植物病原真菌

Determination of the Antifungal Activity of Agricultural Antibiotics 702
ZHOU Yun,ZHANG Zhi-ping,TU Xiao-rong,TU Guo-quan.Determination of the Antifungal Activity of Agricultural Antibiotics 702[J].Acta Agriculturae Universitis Jiangxiensis,2009,31(6).
Authors:ZHOU Yun  ZHANG Zhi-ping  TU Xiao-rong  TU Guo-quan
Abstract:To explore the antifugal activity of agricultural antibiotic 702.With agricultural antibiotic 702 as the test material,and by using mould,yeast,together with 14 kinds of plant pathogenic fungi as the tested microorganism.the inhibitory action of the agricultural antibiotic 702 against all the above microorganism were investigated by the mycelial growth rate method and the hyphae dry weight method.The minimum inhibitory concentration(MIC)(mg/L)of agricultural antibiotic 702 against Aspergillua niger,Penicillium,Mucor,Rhizopus,Trichoderma,Saccharomyces cerevisiae,Candida utilis,Rhizoctonia solani,Magnaporthe grisea and Ustilaginoidea virens respectively were 3.3,6.5,6.5,3.3,3.3,6.5,3.3,23.8,13.4 and 475.4,respectively.The EC_(50_)(mg/L)and EC_(90)(mg/L)of agricultural antibiotic 702 Oil the 14 tested plant pathogenic fungi were as follows:Sclerotinia sclerotiorum,0.23 and 1.95,Colletotrichum gloeosporioides,0.27 and 2.91,Plantain head blight,0.33 and 8.58,Ahernaria alternate,0.89 and 12.02,Plantain sclerotiniose,1.23 and 4.02,Gib-berella zeae,1.40 and 7.38,Verticillium dahliae,1.53 and 5.07,Colletotrichum sp.,1.73 and 5.61,Rhizoctonia solani,1.89 and 7.03,Gloeosporium sp.,3.21 and 14.17,Fusarium solani,3.40 and 20.78,Alternaria sp.,4.13 and 22.01,Magnaporthe grisea,12.51 and 29.36,Ustilaginoidea virens,59.42 and 201.80.Agricultural antibiotics 702 had a powerful antifungal activity,which was useful for the application of the agricultural antibiotic 702.
Keywords:Streptomyces 702  agricultural antibiotic 702  antifungal activity  plant pathogenic fungi
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