| 牛MBL1基因启动子区单核苷酸多态性 |
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| 引用本文： | 刘建博,鞠志花,李秋玲,马丽娟,仲跻峰,王长法.牛MBL1基因启动子区单核苷酸多态性[J].中国农业科技导报,2011,13(2):38-45. |
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| 作者姓名： | 刘建博 鞠志花 李秋玲 马丽娟 仲跻峰 王长法 |
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| 作者单位： | (1.吉林农业大学中药材学院, 长春 130118,2.山东省农业科学院奶牛研究中心, 济南 250100,3.吉林农业科技学院, 吉林 132101) |
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| 基金项目： | 国家863计划项目(2006AA10Z1D92007AA10Z169); 现代农业产业技术体系(nycytx-0107); 山东省良种工程项目(2007LZ015-06); 山东省农业科学院创新基金(2006YCX028)资助 |
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| 摘 要： | 为研究牛甘露糖结合凝集素1(mannose-binding lectin 1, MBL1)基因启动子区单核苷酸多态性,采用聚合酶链式反应限制片长多态性(restriction fragment length polymorphism, RFLP)、单链构象多态性(single-strand conformation polymorphism, SSCP)及直接测序的方法检测了1 073头中国荷斯坦奶牛、69头鲁西黄牛和24头渤海黑牛启动子区-2194(A>C)、-1446(T>C)、-1330(G>A)三个位点的不同基因型分布。结果表明,在-2194位点和-1330位点处渤海黑牛只检测到两种基因型(AA/AC,GG/GA);而鲁西黄牛群体中在-1330位点处没有检测到AA型。三个品种牛群在该三个位点的优势等位基因相同,分别为A、C、G,频率为A(CH 0.900 1/LYC 0.760 9/BBC 0.937 5 )、C(CH 0.604 8/LYC 0.768 1/BBC 0.625)和(CH 0.725 1/LYC 0869 6/BBC 0.895 8)。三个品种牛的优势等位基因相对保守,需进一步研究分析这3个多态位点与牛生产性状的关系。
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| 关 键 词： | 牛 MBL1基因 启动子区 单核苷酸多态性 |
Single Nucleotide Polymorphism in the Promoter Region of MBL1 Gene in Bos Taurus |
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| LIU Jian-bo,JU Zhi-hua,LI Qiu-ling,MA Li-juan,ZHONG Ji-feng,WANG Chang-fa.Single Nucleotide Polymorphism in the Promoter Region of MBL1 Gene in Bos Taurus[J].Journal of Agricultural Science and Technology,2011,13(2):38-45. |
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| Authors: | LIU Jian-bo JU Zhi-hua LI Qiu-ling MA Li-juan ZHONG Ji-feng WANG Chang-fa |
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| Institution: | (1.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118|2. Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan 250100|3.Jilin Agricultural Science and Technology College, Jilin 132101, China) |
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| Abstract: | In order to study the single nucleotide polymorphisms(SNPs) in the promoter region of Mannose-binding Lectin1,the genotypes of 3 loci at-2194(A>C),-1446(T>C),-1330(G>A) in the promoter region of MBL1 were detected by RFLP(restriction fragment length polymorphism),SSCP(single-strand conformation polymorphism) and directly sequencing method,with 1 073 Chinese Holstein cattles,69 Luxi Yellow cattle and 24 Bohai Black cattles as materials.The results showed that only 2 genotypes(AA/AC,GG/GA) were observed at si... |
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| Keywords: | Bos taurus MBL1 gene promoter region single nucleotide polymorphisms(SNPs) |
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