Diagnosis of proliferative enteritis in frozen and formalin-fixed,paraffin-embedded tissues from a hamster,horse, deer and ostrich using a Lawsonia intracellularis-specific multiplex PCR assay |
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| Institution: | 1. Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium;2. Small Animal Department, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium;1. Neurosurgical Clinic, Goethe-University Frankfurt, Germany;2. Medical Clinic II, Goethe-University Frankfurt, Germany;3. Institute for Neuroradiology, Goethe-University Frankfurt, Germany;1. Gastroenterology and Liver Services, Bankstown Hospital, Sydney South West Local Health District, Sydney, New South Wales, Australia;2. Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia;3. Department of Histopathology, Douglass Hanly Moir Pathology, Sydney, New South Wales, Australia;4. Faculty of Medicine, The University of Notre Dame, Sydney, New South Wales, Australia;5. Ingham Institute of Applied Medical Research, Sydney, New South Wales, Australia;6. Immunology Division, The Garvan Institute of Medical Research, Sydney, New South Wales, Australia;7. Australian School of Advanced Medicine, Macquarie University, Sydney, New South Wales, Australia;1. Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan;2. Department of Radiology, Nagoya University Graduate School of Medicine, Nagoya, Japan;3. Department of Thoracic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan;4. Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan;1. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia;2. Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia;3. Australian Centre for Research on Separation Science, School of Physical Sciences − Chemistry, University of Tasmania, 7001 Hobart, Tasmania, Australia;1. Department of Anatomy and Embryology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan;2. Laboratory of Veterinary Anatomy, Faculty of Applied Biological Sciences, Gifu University, Yanagido, Gifu, Japan;3. Fukui Prefectural Dinosaur Museum, Fukui, Japan;4. Department of Human Anatomy, Oita University Fuculty of Medicine, Yufu, Oita, Japan;5. Medical Education Center, Ehime University Graduate School of Medicine, Toon, Ehime, Japan |
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| Abstract: | Proliferative enteritis (PE) is an enteric disease that has been reported in a variety of animals. It is caused by an obligate intracellular bacterium identified in swine as Lawsonia intracellularis. The organism can be detected ante-mortem in swine with PE using molecular diagnostic methods. The disease can be diagnosed post-mortem in all species by gross examination of tissues and special histologic staining procedures. In this study we extracted total DNA from frozen or formalin-fixed, paraffin-embedded tissues from cases of pig, hamster, horse, deer and ostrich PE. The samples were subjected to a multiplex PCR reaction using primers specific for a swine isolate of L. intracellularis. Identical sized PCR products were detected in samples from all animals with PE and the specificity of the PCR reaction for L. intracellularis was demonstrated by Southern-blotting and hybridization using specific probes. These results suggest that the intracellular organism of PE in these species are all very closely related to the causative agent of PE in swine, L. intracellularis. In addition, this multiplex PCR assay can be used to detect the organism in frozen or archival tissues, facilitating retrospective diagnosis of PE. |
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