Structural features of a new heteropolysaccharide from the fruit bodies of Melia azedarach and its effect on cytotoxic activity |
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| Authors: | Liang He Ning Yin Jun-Wen Cheng Xue-Qian Wu Jian-Xin Jiang Xian-Liang Song |
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| Affiliation: | aDepartment of Natural Products Chemistry, Institute of Biological Technology, Zhejiang Forestry Academy, 310023 Hangzhou, China;bThe Key Laboratory of Biochemical Utilization of Zhejiang Province, 310023 Hangzhou, China;cChemical Engineering of Forestry Products, College of Material and Science Technology, Beijing Forestry University, 10083 Beijing, China |
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| Abstract: | Water-soluble polysaccharides were extracted from the fruit pulps of Melia azedarach and some columns were undertaken to isolate the major polysaccharide (MPS-III). Its structural features were elucidated by IR analysis, carbohydrate analysis, periodate oxidation, Smith degradation, methylation analysis and NMR spectroscopy. Then it was evaluated for the cytotoxic activity in vitro against four human cell lines, using the sulphorhodamine B assay. The data obtained indicated that MPS-III contains a α(1→4) main chain backbone composed of arabinose, mannose in a molar ratio of 1.31:1.0 and has α(1→6) branch structure. And MPS-III showed a strong cytotoxic activity in the BGC-823 cell line. |
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| Keywords: | Melia azedarach Heteropolysaccharide Structural analysis Cytotoxic activity |
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