| 红豆杉体外培养生产紫杉醇研究 |
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| 引用本文： | 李云,梁庆丰,曹孜义.红豆杉体外培养生产紫杉醇研究[J].北京林业大学学报,2003,2(1):86-92. |
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| 作者姓名： | 李云 梁庆丰 曹孜义 |
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| 作者单位： | 1. 北京林业大学生物科学与技术学院
2. 甘肃农业大学农学院 |
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| 基金项目： | 引进国际先进农业科技计划(948计划);201044; |
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| 摘 要： | 该文从培养基组成、培养条件、培养基中不同添加物对红豆杉细胞培养和细胞中紫杉醇产量的影响 ,以及在提高紫杉醇产量中有关酶代谢调节、细胞株系选择和培养方法的改进等方面 ,着重介绍了近几年来的研究成果 .同时在毛状根培养、冠瘿瘤组织培养、水培根培养和瘤培养等培养材料的扩展方面 ,对国内外所作的尝试性研究和新的报道进行了综述 .国内外学者围绕红豆杉体外培养方法优化、提高紫杉醇产量等问题进行了许多尝试和深入细致的研究工作 ,取得了令人瞩目的进展 ,为利用红豆杉体外培养进行紫杉醇商业化生产提供了可能
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| 关 键 词： | 红豆杉 紫杉醇 细胞工程 |
Recent advances of studying on Taxus cell engineering |
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| Li Yun, Liang Qingfeng, Cao Ziyi. School of Biological Sciences and Biotechnology,Beijing For. Univ.,P.R.China..Recent advances of studying on Taxus cell engineering[J].Journal of Beijing Forestry University,2003,2(1):86-92. |
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| Authors: | Li Yun Liang Qingfeng Cao Ziyi School of Biological Sciences and Biotechnology Beijing For Univ PRChina |
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| Institution: | Li Yun, Liang Qingfeng, Cao Ziyi. School of Biological Sciences and Biotechnology,Beijing For. Univ.,100083,P.R.China. |
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| Abstract: | This paper reviews the effects of medium composition, culture condition, medium accretion, regulation enzyme, selection of improved Taxus cell, and optimization method on the maximum biomass in cell culture and the increase of the taxol production from the latest reports. Hairy root cultures of Taxus plants, roots of hydroponically grown Taxus plants and nodule cultures of Taxus plants are mainly introduced about the enlargements of cultural materials. Improving methods of Taxus tissue and cell culture in vitro and increasing of the taxol yield have been successfully studied at home and abroad. The achievements in the above mentioned fields provide possibilities to produce taxol commercially by using Taxus tissue and cell culture in vitro. |
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| Keywords: | Taxus taxol cell engineering |
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