| 应用清蛋白PAGE技术进行玉米自交系类群划分的初步研究 |
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| 引用本文： | 陈景堂; 池书敏; 刘志增; 孟义江; 祝丽英,马占元.应用清蛋白PAGE技术进行玉米自交系类群划分的初步研究[J].玉米科学,2001,9(2):018-021. |
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| 作者姓名： | 陈景堂; 池书敏; 刘志增; 孟义江; 祝丽英 马占元 |
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| 作者单位： | 1. 河北农业大学农学院,保定071001
2. 河北省农林科学院,石家庄 050051 |
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| 基金项目： | 本研究得到河北省农业厅资助 |
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| 摘 要： | 利用改进的聚丙烯酰胺凝胶电泳技术,对12个玉米自交系子粒清蛋白进行分析,结果共分离出24条谱带,其中公共谱带4条,占16.7%,多态性谱带20条,占83.3%,平均每个自交系分离出1.7条多态性谱带.通过电泳谱带进行聚类,可将12个自交系分为4大类:第Ⅰ类为478、8112、冀815和冀53;第Ⅱ类为黄早4、502、冀35、京404、81515和52106;第Ⅲ类为丹340;第Ⅳ类为Mo17.聚类结果与已知系谱来源基本吻合,表明改进的聚丙烯酰胺凝胶电泳技术在玉米自交系类群划分上的应用是基本可行的.
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| 关 键 词： | 玉米自交系 清蛋白 PAGE 类群划分 |
| 文章编号： | 1005-0906(2001)02-0018-04 |
| 收稿时间： | 2000/12/31 0:00:00 |
| 修稿时间： | 2000年12月31 |
Heterotic Grouping of Maize Inbred_line with PAGE Techniques |
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| CHEN Jing-tang,CHI Shu-min,LIU Zhi-zeng,MENG Yi-jiang,ZHU Li-ying and MA Zhan-yuan.Heterotic Grouping of Maize Inbred_line with PAGE Techniques[J].Journal of Maize Sciences,2001,9(2):018-021. |
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| Authors: | CHEN Jing-tang CHI Shu-min LIU Zhi-zeng MENG Yi-jiang ZHU Li-ying and MA Zhan-yuan |
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| Abstract: | Electrophoretic analysis was made on the seed albumins of 12 maize inbred lines by improved polyacryamide gel electrophoresis(PAGE)techniques.24 electrophoretic bands consisting of 4 common bands (16.7%)and 20 polymorphic bands(83.3%)were exhibited.On average,1.7 polymorphic bands could be detected from one maize inbred line.12 inbred lines could be clustered and classified into four groups based on electrophoretic bands.Group I includes Huangzao4,502,Ji35,Jing404,81515 and 52106.Group Ⅱ comprises 478,8112,Ji815 and Ji53.Dan340 belongs to group Ⅲ and Mo17 is in group Ⅳ.The four groups by cluster analysis with PAGE conform to the pedigree method.The results of study indicated that it is practical to group maize inbred lines with PAGE techniques. |
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| Keywords: | Inbred line maizel Albumin PAGE Cluster analysis |
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