| 小美石斛茎段培养的研究 |
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| 作者姓名： | 林泋君 |
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| 作者单位： | 云南农业职业技术学院 昆明650031 |
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| 基金项目： | 云南农业职业技术学院校科研和教改项目 |
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| 摘 要： | 用小美石斛茎段作外殖体,进行试管繁殖。筛选出各培养阶段适宜的培养基,(1)腋芽萌生MS 6-BA5mg/L NAA0.4mg/L;(2)继代增殖MS 6-BA2mg/L NAA0.2mg/L,三种培养基MS,1/2MS,N6,以MS增殖效果最好,(3)适宜的生根培养基为1/2MS NAA0.1mg/L PP3330.1mg/L其中平放的芽块生根速度,生根质量和生根率均高于直立放置的。
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| 关 键 词： | 小美石斛 茎段培养 |
Stem-segment Culture and Tube Propagation of Dendrobium bellatulum Rolfe |
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| Authors: | Lin Huijun |
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| Institution: | Y unnan Agriculture Vocational technical college, Kunming 650031 |
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| Abstract: | The stem-segment with axillary buds of Dendrobium bellatulum Rolfe as the explants, were cultured in tubes. The results of experiments suggested that appropriate media for respective culture stages were: (1) The germinaton of axillary buds: Ms 6-BA5mg/L NAA0.4mg/L (2) The differentiation and subculture Ms 6-BA2 mg/L NAA0.2 mg/L (3)Rootage: 1/2 MS NAA0.2mg/L PP333 was optional basal medium for proliferation , the rooting speed, number and percentage of adventitionous buds placed the medium levelly were significantly higher than thouse placed uprightly. |
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| Keywords: | Dendrobium bellatulum Rolfe Stem-segment culture Tube propagation |
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