| 君子兰转化酶基因片段(CMCW1)的克隆和序列分析 |
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| 引用本文： | 汪琼,王燕,程水源,费永俊,金卫斌.君子兰转化酶基因片段(CMCW1)的克隆和序列分析[J].园艺学报,2004,31(2):256-259. |
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| 作者姓名： | 汪琼 王燕 程水源 费永俊 金卫斌 |
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| 作者单位： | ( 扬州大学园艺系，扬州 225009； 湖北农学院园艺系，荆州 434025； 湖北农学院湖北省涝渍灾害与湿地农业重点实验室，荆州 434025) |
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| 基金项目： | 湖北省涝渍灾害与湿地农业重点实验室开放基金资助项目(HNKFJ2002B03) |
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| 摘 要： | 分析植物酸性转化酶基因的保守区序列，设计一对PCR引物，以君子兰基因组DNA为模板，采用PCR方法扩增出长约500 bp的DNA片段，克隆入pGEM-TEasy载体，测序结果表明获得君子兰酸性转化酶基因家族的一个成员CMCW1，该基因片段长518 bp，不含内含子，编码172个氨基酸。其序列已在GenBank中登记(登记号为AY151269)。在GenBank中进行同源性检索的结果表明，该成员编码的氨基酸与其它植物细胞壁酸性转化酶编码的氨基酸同源性较高。
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| 关 键 词： | 君子兰 转化酶 基因 克隆 |
| 文章编号： | 0513-353X(2004)02-0256-03 |
| 收稿时间： | 2003-4-24 |
| 修稿时间： | 2003-7-28 |
Molecular Cloning and Sequence of Acid Invertase Gene Fragments(CMCW1)from Clivia miniata |
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| Wang Qiong,Wang Yan,Cheng Shuiyuan,Fei Yongjun,and Jin Weibin.Molecular Cloning and Sequence of Acid Invertase Gene Fragments(CMCW1)from Clivia miniata[J].Acta Horticulturae Sinica,2004,31(2):256-259. |
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| Authors: | Wang Qiong Wang Yan Cheng Shuiyuan Fei Yongjun and Jin Weibin |
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| Institution: | 1. Department of Endocrinology, Chengdu 610083, Chin;
2. Department of Neurosurgery, Chengdu 610083, Chin;
3. Department of Laboratory, Chengdu Military Command General Hospital, Chengdu 610083, China;
4. Chengdu Bai'ao Biol-Tech Limited Company, Chengdu 610026, China |
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| Abstract: | A pair of primers, designed from conserved regions of plant acid invertase genes, was used to amplify same gene by polymerase chain reaction ( PCR). As a result, one 518 bp fragment was obtained, and then cloned into pGEM -T Easy and sequenced. It has no intron and encoded 172 amino acids. This gene has been registered in GenBank with accession number AY151269. The deduced amino acid sequence is higher identical to the gene from other plant. |
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| Keywords: | Clivia miniata Invertase Gene Cloning |
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