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| 葫芦种子传黄瓜绿斑驳花叶病毒的检测* | |
| 引用本文： | 秦碧霞,蔡健和,陆秀红,林林,廖富荣,刘志明. 葫芦种子传黄瓜绿斑驳花叶病毒的检测*[J]. 植物保护, 2011, 37(3): 109-112. DOI: 10.3969/j.issn.0529-1542.2011.03.023 |
| 作者姓名： | 秦碧霞 蔡健和 陆秀红 林林 廖富荣 刘志明 |
| 作者单位： | 1. 广西农业科学院植物保护研究所,南宁,530007;广西作物遗传改良生物技术重点开放实验室,南宁,530007 2. 广西农业科学院植物保护研究所,南宁,530007 3. 广西玉林市农业科学研究所,玉林,537000 4. 厦门出入境检验检疫局,厦门,361012 |
| 基金项目： | 广西科科学基金项目，国家质检总局科技计划项目 |
| 摘 要： | 从感染黄瓜绿斑驳花叶病毒（Cucumber green mottle mosaic virus, CGMMV）的葫芦植株上收取种子，通过苗期症状观察法、双抗体夹心酶联免疫吸附法（DAS-ELISA）、免疫捕获反转录PCR（IC-RT-PCR）法测定葫芦种子的带毒情况，并用生物学接种方法测定葫芦种子携带病毒的侵染活性。苗期症状观察法结果表明，199株幼苗有2株表现花叶斑驳症状，种子传毒率为1.01%；而利用DAS-ELISA和IC-RT-PCR法随机检测30粒葫芦病株种子，CGMMV检出率为100%。种子各部位携带的CGMMV接种葫芦表现典型的花叶斑驳症状，表明葫芦种子携带的CGMMV具有侵染活性。DAS-ELISA检测葫芦种子CGMMV的灵敏度为1/5120种子研磨液。 |
| 关 键 词： | 黄瓜绿斑驳花叶病毒 种子带毒 检测 |
Detection of the Cucumber green mottle mosaic virus in seeds of bottle gourd |
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| Qin Bixi,Cai Jianhe,Lu Xiuhong,Lin Lin,Liao Furong,Liu Zhiming. Detection of the Cucumber green mottle mosaic virus in seeds of bottle gourd[J]. Plant Protection, 2011, 37(3): 109-112. DOI: 10.3969/j.issn.0529-1542.2011.03.023 | |
| Authors: | Qin Bixi Cai Jianhe Lu Xiuhong Lin Lin Liao Furong Liu Zhiming |
| Affiliation: | 1. Institute of Plant Protection, Guangxi Academy of Agricultural Sciences, Nanning530007, China;2. Guangxi Key Laboratory of Crop Genetic Improvement and Biotechnology, Nanning530007, China;3. Yulin Research Institute of Agricultural Sciences, Yulin537000, China;4. Xiamen Entry Exit Inspection and Quarantine Bureau, Xiamen361012, China |
| Abstract: | The seed transmission rate of Cucumber green mottle mosaic virus(CGMMV) in the bottle gourd Lagenaria siceraria was detected based on seedling symptoms,DAS-ELISA and immunocapture RT-PCR(IC-RT-PCR).Seed transmission ratio of CGMMV in bottle gourd from the virus-contaminated seeds was 1.01%,while seed-borne ratio of CGMMV in bottle gourd seeds tested was 100% by DAS-ELISA and IC-RT-PCR.CGMMV in bottle gourd seeds was demonstrated to have infection activity by biological inoculation.The sensitivity of DAS-ELI... |
| Keywords: | Cucumber green mottle mosaic virus seed-borne virus detection |
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