| 玉米sbe2a基因RNAi载体的构建及转化玉米的初步研究 |
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| 引用本文： | 关淑艳,楚海娇,刘慧婧,刘广娜,刘学志,王丕武.玉米sbe2a基因RNAi载体的构建及转化玉米的初步研究[J].吉林农业大学学报,2011,33(2):172-176. |
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| 作者姓名： | 关淑艳 楚海娇 刘慧婧 刘广娜 刘学志 王丕武 |
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| 作者单位： | 1. 吉林农业大学生物技术中心,长春,130118
2. 吉林省公主岭市植检站,公主岭,136100 |
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| 基金项目： | 国家转基因专项(20082x08003005),吉林省财政厅项目(200806),吉林省科技成果转化补助项目(20095044) |
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| 摘 要： | 采用PCR技术克隆了玉米淀粉分支酶sbe2a基因的cDNA片段,将其克隆到pMD18-T载体,对重组子进行PCR检测和限制性内切酶分析,并进行序列分析.结果表明:克隆片段长度为562bp,将该基因的正义和反义片段插入到pCAMBIA1301改造过的载体p35-1301的35S启动子下游,构建高效RNAi表达载体,通过花...
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| 关 键 词： | 玉米 淀粉分支酶基因sbe2a RNAi 遗传转化 |
| 收稿时间： | 2010-07-16 |
The Primary Study on Construction of RNAi Expression Vector of sbe2a Gene and Maize Transformation |
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| GUAN Shu-yan,CHU Hai-jiao,LIU Hui-jing,LIU Guang-na,LIU Xue-zhi,WANG Pi-wu.The Primary Study on Construction of RNAi Expression Vector of sbe2a Gene and Maize Transformation[J].Journal of Jilin Agricultural University,2011,33(2):172-176. |
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| Authors: | GUAN Shu-yan CHU Hai-jiao LIU Hui-jing LIU Guang-na LIU Xue-zhi WANG Pi-wu |
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| Institution: | GUAN Shu-yan1,CHU Hai-jiao1,LIU Hui-jing1,LIU Guang-na1,LIU Xue-zhi2,WANG Pi-wu11.Biotechnology Center of Jilin Agricultural University,Changchun 130118,China,2.Gongzhuling Plant Protection Quarantine Station,Gongzhuling 136100 |
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| Abstract: | cDNA segment of corn starch branching enzymes gene sbe2a was cloned by PCR technique and inserted into pMD18-T vector.The recombinant clone was detected by PCR and the sequence and fragments of restriction enzymes were analyzed.The results showed that the length of the clone sequence was 562 bp.The sense and anti-sense fragments of clone sequence were inserted into downstream of 35S promoter of pCAMBIA1301 to construct over-expression RNAi vector.And then it was transformed into corn inbred lines TIE 7922 b... |
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| Keywords: | maize starch branching enzyme gene sbe2a RNAi genetic transformation |
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