草莓乙烯受体Ers1基因反义植物表达载体构建

目的]构建草莓Ers1基因反义植物表达载体,为探明该基因的功能以及采用生物技术方法改良草莓品种奠定基础。方法]根据已克隆的草莓乙烯受体Ers1基因序列及表达载体pBI121上的酶切位点设计1对带限制性内切酶位点的特异性引物,以Ers1基因的测序质粒为模板,PCR扩增到草莓Ers1基因片段,克隆片段及载体经双酶切消化后,回收产物定向连接,将克隆片段反向补插入到植物表达载体pBI121的35S和gusA之间,构建成该基因的反义植物表达载体。结果]经酶切鉴定,成功构建了草莓Ers1基因反义植物表达载体。结论]该研究为进一步探讨Ers1基因功能及利用生物技术方法调控果实成熟衰老进程,从而改善草莓果实的贮运性能奠定基础。

Expression Vector Construction of Strawberry Ers1 Gene

Objective] To construct the antisense expression vector of strawberry Ers1 gene,in order to explore the function of the gene and use the biological technology to improve the strawberry cultivars.Method] According to the restriction enzyme sites of expression vector and cloning sequence of strawberry Ers1 gene,one pair of primers containing restriction enzyme sites were designed and used to amplify sequenced plasmid.PCR product and the plasmid pBI121 were digested by the corresponding restricted enzymes respectively,and linked directionally.Then the antisense expression vector can be designed.Result] Through restriction enzyme identification,the antisense plant expression vector of strawberry Ers1 gene was successfully constructed.Conclusion] The study laid foundation for further discussing the functions of Ers1 gene,regulating the fruits maturing process via biotechnological method and improving the storage and preservation performance of strawberries.