HPLC法测定榛子雄花中槲皮素和山萘素的含量

[目的]建立榛子雄花中槲皮素和山萘素含量的测定方法。[方法]采用高效液相色谱法测定榛子雄花中槲皮素和山萘素的含量。色谱条件:色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm);无水甲醇-浓度0.4%磷酸溶液(50∶50,V/V)为流动相,流速为1.0ml/min,检测波长为360 nm。[结果]槲皮素在0.022～0.658μg/ml浓度范围内线性关系良好,相关系数r=0.999 9,平均回收率为99.4%,RSD为0.73%;山萘素在0.012 1～0.362 0μg/ml浓度范围内线性关系良好,相关系数r=0.999 9,平均回收率为100.4%,RSD为1.49%;测得各地样品中榛子雄花中槲皮素的含量为0.35～0.58 mg/g,山奈素的含量为0.174～0.512 mg/g。[结论]该方法简单、快速、重现性好,可用于榛子雄花中槲皮素和山萘素的含量测定。

Determination the Content of Quercetin and Kaempferol in Male Flower of Corylus heterophylla Fisch.

[Objective] To establish an HPLC method for determining the content of quercetin and kaempferol in male flower of Corylus heterophylla Fisch.[Method] A HPLC analysis was performed on a Diamonsil C18 column(250 mm×4.6 mm,5 μm) with the detection wavelength at 360 nm.Following the injection of sample solution,a elution with methanol-0.4% phosphoric acid(V/V,50:50) as mobile phase was initiated at a flow rate of 1.0 ml/min.[Result] The quercetin showed a good relationship in the range of 0.022 to 0.658 μg(r=0.999 9).The average recovery of quercetin was 99.4% with the RSD of 0.73%.The kaempferol showed a good relationship in the range of 0.012 1 to 0.362 0 μg(r=0.999 9).The average recovery of kaempferol was 100.4% with the RSD of 1.49%.The content of quercetin in C.heterophylla male flower was 0.35 to 0.58 mg/g and that of kaempferol ranged from 0.174 to 0.512 mg/g.[Conclusion] The method is simple and rapid with a good reproducibility,which can be used to determine the content of quercetin and kaempferol in C.heterophylla male flower.