车前种质资源遗传多样性ISSR分析

[目的]研究江西、湖南、湖北等7个省份的车前种质资源的遗传多样性。[方法]采用ISSR技术,对28份车前样品进行遗传多样性和亲缘关系分析。[结果]从40条引物中共筛选出16条可用于车前遗传多样性分析的ISSR引物,共扩增出131条带,其中多态性条带(PPB)有107条,占81.7%。ISSR数据分析显示,多态性位点百分率(P)为75.3%;平均等位基因观测数为0.071 3;有效等位基因数为0.299 0;Nei,s基因多样性指数(H)为0.360 1;Shannon多态性信息指数(I)为0.535 4。[结论]车前种质资源遗传多样性的地理差异较为明显;野生种与栽培种基因型差异较大。

ISSR Analysis on Genetic Diversity of Plantago asiatica L. Germplasm Resource

[Objective] To study the genetic diversity of Plantago asiatica L.germplasm resource from seven provinces such as Jiangxi Province,Hunan Province and Hubei Province.[Method] ISSR technology was used to analyze the genetic diversity and genetic relationship of 28 copies of P.asiatica samples.[Result] Sixteen primers that were selected at total forty primers were used to analyze the genetic diversity.131 strips were amplified,among which 107 strips had polymorphism,accounting for 81.7% of the total amount.ISSR data showed that the scale of diversity point(P) was 75.3%,observed number of alleles was 0.071 3,effective number of alleles was 0.299 0,Nei′s gene diversity(H) was 0.360 1,Shannon’s Information index(I) was 0.535 4.[Conclusion] The genetic diversity of P.asiatica germplasm resource had great difference at geography.The wild varieties and cultivated varieties had great difference at gene type.