PEG胁迫下甘蓝型油菜品种（系）种子发芽耐旱性鉴定

以10%（w/v）PEG6000做渗透介质模拟干旱条件，对不同来源的甘蓝型油菜(Brassica napus L.)品种（系）进行干旱胁迫下种子发芽鉴定，分析油菜种子发芽耐旱性的遗传多样性。结果表明：干旱胁迫下，部分材料处理与对照的发芽性状有显著性差异，81个甘蓝型油菜材料的平均苗高处理比对照低36.5％，平均鲜重处理比对照低31％，成苗率降低11.1％。甘蓝型油菜品种（系）耐旱性有较大遗传差异，幼苗活力指数的变异幅度为19％－81%，平均活力指数为55％。相关分析表明，活力指数与胁迫下的发芽性状达极显著正相关，可以作为油菜种子发芽耐旱的综合指标。根据活力指数，将81个油菜资源划分为强，中，弱三个耐旱等级，并筛选到30295，30253，中双6号，30703，30296，30290，30704，中油杂9号，德油4号等一批耐旱遗传资源。

Evaluation of drought tolerance in rapeseed(Brassica napus L.)during germination under PEG6000 stress

Drought tolerance of 81 varieties of rapeseeds(Brassica napus L.) from different genetic resources was investigated during germination with simulate water stress by 10%(w/v) PEG6000.The results showed that the seedling traits of several treatments were significantly different from control after drought stress.The average seedling height of treatment deceased 36.5%,the average fresh weight decreased 31% and relative germination rate decrease 11.1%.There were significant difference of drought tolerance among different varieties or lines.The seedling vigor index ranges from 19% to 81%,with an average of 55%.Correlation analyses indicated that the vigor index has significant positive correlation with the seedling traits under drought stress.Some varieties or lines,30295,30253,Zhongshuang 6,30703,30296,30290,30704,Zhuongyouza 9 and Deyou 4,could be classified as high drought tolerant germplasm resources with higher vigor index.