| 提高苏云金素合成纯度的发酵工艺的优化 |
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| 引用本文： | 刘要,阮丽芳,刘晓艳,曹诗云,孙明.提高苏云金素合成纯度的发酵工艺的优化[J].农业生物技术学报,2010,18(2):406-412. |
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| 作者姓名： | 刘要 阮丽芳 刘晓艳 曹诗云 孙明 |
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| 作者单位： | 华中农业大学生命科学技术学院,农业微生物学国家重点实验室,武汉,430070 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，国家林业局"948"引进项目 |
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| 摘 要： | 苏云金芽胞杆菌中华亚种CT-43菌株(Bacillus thuringiensis subsp.chinensis)是高产苏云金素(简称Thu)的野生菌株.但在产生Thu时总伴随有其他代谢物(简称ZZ)的合成.而且ZZ很难与Thu分离开.本实验通过比较不同的培养基,得到G培养基能抑制菌株CT-43合成ZZ.然后利用分批补料发酵的方法,通过正交试验设计,进一步抑制ZZ的合成,并提高Thu在有机萃取物中的含量.得到优化后的发酵工艺是:菌株CT-43在G培养基中培养至第20 h,同时补柠檬酸钠和葡萄糖至终浓度分别为2.0 g/L和16 g/L,再继续发酵至第52 h.通过此发酵工艺制备的Thu峰面积占初提物总峰面积的74.84%,比用LB培养基发酵所得提取物的29.85%提高了两倍多.为得到高纯度的Thu和更深入的研究创造了条件.最后通过液质联用技术证实,发酵工艺优化后,菌株CT-43发酵上清液中主要物质是Thu,几乎检测不到ZZ,而且优化后的Thu分子量并没有改变.
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| 关 键 词： | 苏云金芽胞杆菌 苏云金素 补料发酵 正交试验设计 纯化 液质联用 |
The Optimization of Fermentation Process to Increase Purity of Thuringiensin in the Extract from Supernatant Liquid |
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| Liu Yao,Ruan Lifang,Liu Xiaoyan,Cao Shiyun,Sun Ming.The Optimization of Fermentation Process to Increase Purity of Thuringiensin in the Extract from Supernatant Liquid[J].Journal of Agricultural Biotechnology,2010,18(2):406-412. |
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| Authors: | Liu Yao Ruan Lifang Liu Xiaoyan Cao Shiyun Sun Ming |
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| Institution: | Liu Yao Ruan Lifang Liu Xiaoyan Cao Shiyun Sun Ming State Key Laboratary of Agricultural Microbiology,College of Life Science & Technology,Huazhong Agricultural University,Wuhan,430070 |
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| Abstract: | Bacillus thuringiensis subp.chinensis strain CT-43 highlyproduces thuringiensin(Thu).Meanwhile, other metabolizing product(ZZ) was synthesized, but was difficultly removed from Thu.In this study, various media were screened and the G medium was found to inhibit the ZZ synthesis.Based on the G medium, feed-batch fermentation and orthogonal arrays were combined to further inhibit ZZ synthesis and increase the yield of Thu.The optimal fermentation process was that, after strain CT-43 was cultured in G medium f... |
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| Keywords: | Bacillus thuringiensis Thuringiensin Feed-batch fermentation Orthogonal arrays Purifying HPLC-MS |
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